辛伐他汀对去势大鼠骨质疏松防治作用的试验研究

目的:观察辛伐他汀对去势大鼠骨质疏松防治的作用.方法:40只雌性SD大鼠,随机平均分为4组,即假手术组、去势对照组、雌激素组(苯甲酸雌二醇0.1mg/kg,每三天皮下注射一次,连续用药至动物处死)、辛伐他汀组(辛伐他汀5mg/(kg.d)灌胃连用2周,停用5周,再连用2周,停药处死动物).术后一个月开始药物干预.试验开始及处死动物后分别测定大鼠股骨的骨密度,放射免疫法测定IL-6、BGP的值,光镜下观察大鼠股骨病理学变化.结果:试验结束时,去势对照组大鼠骨密度BMD低于假手术组,雌激素组、辛伐他汀组的BMD高于去势对照组,均P＜0.05.去势对照组的IL-6的值高于假手术组,治疗组IL-6的值低于去势对照组,而雌激素组IL-6的值低于辛伐他汀组,均P＜0.05.去势对照组的BGP高于假手术组,而治疗组的BGP高于去势对照组,辛伐他汀组的BGP高于雌激素组,均P＜0.05.治疗组与去势对照组相比骨组织病理学变化均有改善.结论:辛伐他汀能增加骨质疏松大鼠的骨密度,改善骨组织病理学变化,提高BGP的水平,降低IL-6的水平,有效的防治骨质疏松.

Study on prevention and therapeutic effects of simvastatin on osteoporosis in rats

Objective:To observe the prevention and therapeutic effects of simvastatin on the osteoporosis in rats.Methods:Forty SD female rats were randomly divided into four groups:sham operation group,OVX group,estrogen group and simvastatin group.One month after OVX operation,we began medicine intervention.Estrogen group was subcutaneously injected EB(0.1mg/kg)per triduum until being sacrificed.Simvastatin group was intragastrically administered simvastatin 5mg/(kg.d)for 2 weeks,then the administration was stopped for 5 weeks and then continued for 2 weeks until being sacrificed.After the intervention,we observed the change of rats' BMD,bone histopathology and expressions of IL-6 and BGP.Results:The level of BMD in OVX group was lower than that in the sham group(P<0.05).The level of BMD raised in treated groups compared to the OVX group(P<0.05).The expression of IL-6 in OVX group raised compared to the sham group(P<0.05).The expression of IL-6 in all treated groups degraded compared to the OVX group(P<0.05),especially in the estrogen group.The expression of BGP in OVX group raised compared to the sham group(P<0.05).The expression of BGP in all treated groups,especially the simvastatin group,significantly raised compared to the OVX group(P<0.05).The change of bone histopathology in treated groups was ameliorated compared to the OVX group.Conclusion:Simvastatin could effectively prevent and cure osteoporosis by elevating BMD,ameliorating the bone histopathology,raising the expression of BGP and degrading the expression of IL-6.