Characterization of the luteinizing hormone beta (LH-beta) subunit gene in the Indian river buffalo (Bubalus bubalis) |
| |
Authors: | Basavarajappa Mallikarjuna Shivapura De Sachinandan Thakur Manish Datta Tirtha Kumar Dogra Gaurav Yadav Paras Goswami Surender Lal |
| |
Affiliation: | Animal Biotechnology Center, National Dairy Research Institute, Karnal 132001, Haryana, India. |
| |
Abstract: | The cDNA sequence of leuteinizing hormone (LH) beta subunit was determined to understand the molecular basis for silent oestrus behavior and poor response to superovulation in buffalo. The LH-beta cDNA contains an open reading frame 426 bp long. The deduced sequence of the LH-beta is 141 amino acids in length. The amino acid sequences of the Indian river buffalo LH-beta subunit showed overall similarity to those of other mammals. The nucleotide sequence variability of LH-beta was studied in more than approximately 350 Indian buffaloes covering five different breeds. The results of the sequence analysis showed that the buffalo LH-beta gene is not highly conserved and non-synonymous mutations are not rare, at least in the samples collected randomly from five different breeds and buffalo populations. A total of seven different variants were obtained. In spite of its crucial role in reproduction, variation of the LH-beta gene was found present in this species. The polymorphisms found were unique in the Indian river buffalo population. |
| |
Keywords: | Buffalo Luteinizing hormone (LH) beta SSCP |
本文献已被 ScienceDirect PubMed 等数据库收录! |