体外研究CpG基序对人食管癌疫苗的佐剂效应

目的:探讨CpG基序在人食管癌Eca-109细胞疫苗研究中的佐剂效应.方法:以Eca-109细胞膜表面小肽刺激DC作为食管癌疫苗,HL-60细胞膜表面小肽刺激DC作为对照疫苗.以CpG基序作为佐剂,以CBPw作为对照佐剂,测定各组的T细胞增殖试验和CTL活性.结果:佐剂组的T细胞活性明显高于其余各组(P＜0.01);人食管癌疫苗组的T细胞活性明显高于其余各组(P＜0.01);与Eca-109细胞匹配的HLA也影响了T细胞活性的发挥.结论:CpG基序是人食管癌疫苗的有效佐剂,HLA对T细胞功能有影响.

Adjuvant Effects of CpG Motif-Containing Oligodeoxynucleotide in vitro on Human Esophageal Tumor Vaccine

Objective: To investigate the adjuvant effects of CpG motif-containing oligodeoxynucleotide (CpG) in vitro on human esophageal cancer vaccine. Methods: Matured dendritic cells ( DC ) activated by peptides on Eca-109 cell membranes were used for human esophageal tumor vaccine and matured DC activated by peptides on HL-60 cell membranes were used for control vaccine. With adjuvant of CpG or the cell wall skeleton of coryne bacterium parvum ( CB Pw) , we analyzed each groups T-cell proliferation and cytotoxic T lymphocyte ( CTL) response. Results: The adjuvant of CpG or CBPw markedly enhanced T-cell proliferation and CTL response (P < 0. 01) ; The groups with human esophageal tumor vaccine had markedly greater T-cell proliferation and CTL response than other groups (P <0. 05) ; The HLA gene locus alleles to match Eca-109 cell gene affected the activity of T cells. Conclusion: CpG may be the optimal adjuvant of human esophageal tumor vaccine and the HLA gene locus may affect T cells function.