Biodegradation Property of Beta‐Tricalcium Phosphate‐Collagen Composite in Accordance with Bone Formation: A Comparative Study with Bio‐Oss Collagen® in a Rat Critical‐Size Defect Model |
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| Authors: | Eiji Kato DDS Jeffery Lemler DDS Kaoru Sakurai DDS PhD Masahiro Yamada DDS PhD |
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| Affiliation: | 1. President, Implant and Tissue Engineering Dental Network‐Tokyo, Tokyo, Japan;2. associate professor, Implantology and Periodontics, New York University College of Dentistry, NY, USA;3. professor and chair, Department of Removable Prosthodontics & Gerodontology, Tokyo Dental College, Chiba, Japan;4. principal investigator, Implant and Tissue Engineering Dental Network‐Tokyo, Tokyo, Japan, and assistant professor, Department of Removable Prosthodontics & Gerodontology, Tokyo Dental College, Chiba, Japan |
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| Abstract: | Purpose: The objective of this study was to compare osteoconductivity and biodegradation properties of an in‐house fabricated beta‐tricalcium phosphate (b‐TCP)‐collagen composite with those of Bio‐Oss Collagen® (Osteohealth, Shirley, NY, USA) using a rat calvarial critical‐size defect model. Materials and Methods: b‐TCP–collagen composite material was fabricated by mixing b‐TCP granules having a particle size of 0.15 to 0.8 mm and 75% porosity, with bovine dermis‐derived soluble collagen sponge. The dry weight ratio of b‐TCP granules‐to‐collagen ratios was 4:1. Bio‐Oss Collagen or the b‐TCP–collagen composite was used to fill a 5.0 mm–diameter calvarial defect in rats. The defects were evaluated by histological and histomorphological analyses of decalcified histological sections with hematoxylin and eosin staining 6 and 10 weeks, respectively, after surgery. Results: The defect implanted with the b‐TCP composite contained immature bone structures with dense connective tissue in contrast to the abundant fibrous tissue, but no trabecular structure was observed within the defect implanted with Bio‐Oss Collagen at 6 weeks postoperatively. Eventually, the defect filled with the b‐TCP composite was covered with dense, continuous, mature bone tissue with complete replacement of the graft material. However, in defects filled with Bio‐Oss Collagen, only dense connective tissue, containing limited amounts of immature trabecular bone and abundant remnant Bio‐Oss particles, was observed. Histomorphological analysis revealed that the b‐TCP composite caused greater tissue augmentation with a larger volume of bone tissue observed in the defect and greater bioabsorption of remnant material than Bio‐Oss Collagen. Conclusion: These results indicated that the b‐TCP composite has greater osteoconductivity and better biodegradation properties than Bio‐Oss Collagen; these properties of the b‐TCP–collagen composite complimented bone formation and remodeling. |
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| Keywords: | alloplast bioabsorption bone regeneration bone substitute socket preservation |
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