| LAK细胞对白血病多药耐药细胞的杀伤作用 |
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| 引用本文： | 王骏,陈宝安,邵泽叶,薛萌,陈苏宁,王立新,李翠萍.LAK细胞对白血病多药耐药细胞的杀伤作用[J].东南大学学报(医学版),1999(4). |
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| 作者姓名： | 王骏 陈宝安 邵泽叶 薛萌 陈苏宁 王立新 李翠萍 |
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| 基金项目： | 铁道部科技基金!资助课题(J98Z028) |
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| 摘 要： | 目的:研究淋巴因子激活的杀伤(LAK) 细胞对白血病多药耐药细胞的杀伤效应。方法:采用改良的四甲基偶氮唑盐(MTT)比色法检测LAK细胞对白血病多药耐药细胞株K562/VCR的杀伤率,并与敏感株K562 相比较。结果:LAK 细胞对K562/VCR 细胞的杀伤率明显高于对K562 细胞的杀伤率,并且当效靶比为40∶1 时,这种杀伤率最高。结论:LAK细胞在体外对白血病多药耐药细胞株具有显著的杀伤作用,提示临床上用LAK细胞治疗多药耐药的白血病是可行的
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| 关 键 词： | 四甲基偶氮唑盐比色法 淋巴因子激活的杀伤细胞 白血病 多药耐药 体外 |
Study on Cytotoxic Effects of Lymphokine Activated Killer Cells to Leukemia Multidrug Resistance Cells |
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| Wang Jun,Chen Baoan,Shao Zeye,Xue Meng,Chen Suning,Wang Lixing.Study on Cytotoxic Effects of Lymphokine Activated Killer Cells to Leukemia Multidrug Resistance Cells[J].Journal of Southeast Univ: Medical Sci Ed,1999(4). |
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| Authors: | Wang Jun Chen Baoan Shao Zeye Xue Meng Chen Suning Wang Lixing |
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| Abstract: | Objective:The experiment was designed to investigate the cytotoxic effects of lymphokine activated killer (LAK) cells to leukemia multidrug resistance (MDR) cells. Methods: The cytotoxic effects of LAK cells on the leukemia MDR cell line K562/VCR were studied by improved 3?(4,5?dimethylthiazole?z?yl)?2,5?diphenyl tetrazolium bromide (MTT) assay, and the result was compared with that of LAK cell to the leukemia parents cell line,K562. Results: The killing effect of LAK cells to K562/VCR is obviously better than that of LAK cells to K562.Conclusion:LAK cells are very effective in killing or eliminating the tumor cells of MDR in vitro . |
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| Keywords: | tetrazolium bromide assay lymphokine-activated killer cells leukemia multidrug-resistance in vitro |
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