PCR 与 DIFA 检测沙眼衣原体的比较研究

用所建立的沙眼衣原体质粒引物聚合酶链反应技术（ＰＣＲ）检测１１６例泌尿生殖道沙眼衣原体，并与直接免疫荧光法（ＤＩＦＡ）进行比较。结果ＰＣＲ阳性３８例，ＤＩＦＡ阳性的３２例中有３１例ＰＣＲ阳性。ＰＣＲ敏感性为９６．９％，特异性为９１．７％。ＰＣＲ阳性者于治疗结束１～２周后复诊２１例，其中４例ＰＣＲ仍然阳性。结果显示，ＰＣＲ可快速、敏感、特异地检测泌尿生殖道沙眼衣原体ＤＮＡ，然而对治疗后短期内复诊患者，似乎不宜仅以ＰＣＲ阳性结果作为重复治疗的依据。

A COMPARISON AND FOLLOW-UP STUDY OF POLYMERASE CHAIN REACTION AND DIRECT IMMUNOFLUORECENCE ASSAY FOR THE DETECTION OF UROGENITAL CHLAMYDIA TRACHOMATIS INFECTION

s A plasmid derived polymerase chain reaction(PCR) assay was developed for the detection of chlamydia trachomatis in urogenital specimens of 116 patient,and the results were compared with that DIFA.33 cases were PCR positive,and Ct DNA were detected in 31 of the 32 cases positive by DIFA.The sensitivity and specificity of PCR were 96.9% and 91.7%,respectively .21 cases positive by pretreatment PCR assay were followed up in 1-2 weeks after the completion of treatment,and 4 of them were still PCR positive.The results show that PCR is a rapid,sensitive and specific diagnostic tool for Ct DNA,however,it seems less appropriate to use PCR positive results as indicators to give repetitive medications for patients shortly after treatments.