口岸出入境人群梅毒感染检测方法比较

目的探讨适合口岸出入境人群检测梅毒的方法。方法采用国产酶联免疫吸附试验（ELISA）试剂与甲苯胺红不加热血清试验（TRUST）和梅毒螺旋体颗粒凝集试验（TPPA）进行血清学方法比较,采用聚合酶链反应（PCR）对梅毒患者生殖道皮损渗出液进行检测,分析其传染性。结果国产ELISA试剂与TPPA检测结果阳性符合率为96%（263/273）,2种方法对273例标本的检测结果差异无统计学意义。而TRUST和TPPA检测结果差异有统计学意义,与ELISA检测结果差异亦有统计学意义。PCR检测29例梅毒患者生殖道皮损渗出液和100例初筛阳性血清标本,生殖道皮损渗出液与临床诊断符合率为100%,血清检测结果均为阴性。结论ELISA可替代TRUST和TPPA作为梅毒感染的筛查方法。PCR不能作为出入境人员梅毒传染性判断的方法。

Comparison of the methods of syphilis detection for exit-entry people

Objective To select the methods of syphilis detection for exit-entry people. Methods Three serologic methods including domestic-made enzyme-linked immunosorbent assay （ELISA） reagents,the toluidine red unheated-serum test （TRUST） and treponema pallidum particle agglutination test （TPPA） reagents were compared. The transudate fluid of the syphilis patient in genital duct was detected by polymerase chain reaction （PCR） for analysis of infectivity. Results The positive coincidence rate of the domestic-made ELISA reagents and the TPPA reagents was 96% （263/ 273）. There was no statistics difference between the two methods. The detecting result of TRUST was statistically different compared with that of TPPA, as well as with ELISA. 29 transudate samples from genital duct of the syphilis patient and 100 positive serum samples were detected by PCR. There was a 100% coincidence rate between the detecting results of the transudate samples and the clinical results. The detecting results of serum were all negative. Conclusions ELISA can be used as the screening method to detect the infection of syphilis instead of TRUST and TPPA. PCR is not a suitable confirmation method to detect the infection of syphilis among the exit-entry people.