大鼠视网膜缺血性再灌注中HIF-1α与SDF-1的表达意义

目的:探讨缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)和基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)在缺血性视网膜疾病及视网膜缺血再灌注(retinal ischemia-reperfusion,RIR)发展中的表达规律及二者的相互关系。方法:应用免疫组织化学的方法分析HIF-1α和SDF-1在视网膜缺血再灌注模型中的表达及其关系。结果:在对照组中HIF-1α和SDF-1未见表达或轻微表达,视网膜缺血性再灌注组0h开始表达,6h表达增强,12h表达最强,24h表达减弱,以后随着时间推移表达逐渐下降。结论:HIF-1α和SDF-1表达与视网膜缺血再灌注中视网膜功能损伤密切相关,同时二者具有协同相关性。

Expression and significance of HIF-1α and SDF-1 in retinal ischemia-reperfusion in rats

AIM:To investigate the expression pattern and correlation of hypoxia-inducible factor-1α(HIF-1α) and stromal cell-derived factor-1(SDF-1) in the ischemic retinopathy and retinal ischemia-reperfusion.·METHODS:In order to analyze the correlation and expression between HIF-1α and SDF-1 in retinal ischemia-reperfusion model,immunohistochemical method was used.·RESULTS:In the control group,HIF-1α and SDF-1 were not expressed or slightly expressed,while that in retinal ischemia-reperfusion group began to express immediately after carotid artery ligation,ascended at 6 hour,reached peak at 12 hour,and decreased gradually after 24 hours.·CONCLUSION:HIF-1α and SDF-1 expression are closely relate to retinal ischemia-reperfusion injury,and have a collaborative relationship.