Raptor、Rictor和磷酸化Akt在日光性角化病、Bowen病及鳞状细胞癌中的表达

目的:检测Raptor、Rictor和磷酸化Akt(p-Akt)在日光性角化病、Bowen病和鳞状细胞癌中的表达。方法:采用免疫组化法检测Raptor、Rictor及p-Akt(Ser473)在20例正常皮肤、20例日光性角化病、20例Bowen病及40例鳞状细胞癌中的表达。结果:Raptor在鳞状细胞癌、Bowen病和日光性角化病中的阳性表达率分别为87.50%、70.00%和60.00%,均高于正常皮肤的25.00%(均P0.05);其中低分化鳞状细胞癌中Raptor的阳性表达率为100%,高于高分化鳞状细胞癌的阳性表达率75%(P0.05)。Rictor在鳞状细胞癌、Bowen病和日光性角化病中的阳性表达率分别为80.00%、70.00%及55.00%,均高于正常皮肤阳性表达率的20%(均P0.05);p-Akt(Ser473)在鳞状细胞癌、Bowen病和日光性角化病中的阳性表达率分别为77.50%、65.00%及50.00%,而正常皮肤阳性表达率为0。鳞状细胞癌、Bowen病和日光性角化病中Rictor的阳性表达水平和p-Akt(Ser473)的阳性表达水平均呈正相关(均P0.05)。结论:Raptor、Rictor和p-Akt(Ser473)的高表达可能与日光性角化病、Bowen病和鳞状细胞癌的发生发展有关。

Expression of Raptor,Rictor and p-Akt in actinic keratosis,Bowen's disease and squamous cell carcinoma

Objective:To detect the expression of Raptor,Rictor and p-Akt in the lesions of actinic keratosis (AK),Bowen's disease (BD) and squamous cell carcinoma (SCC).Methods:Immunohistochemistry was used to detect the expression of Raptor,Rictor,p-Akt (Ser473) in the tissue samples from the patients with AK,BD,SCC and normal human skin.Results:The positivity rates of Raptor in SCC,BD and AK were 87.50%,70.00% and 60.00%,which was significantly higher than that in normal skin (Ps<0.05).The positivity rate was 100% in poorly differentiated squamous cell carcinoma and 75% in well differentiated squamous cell carcinomas,with a significant difference (P<0.05).The positivity rates of Rictor in SCC,BD and AK were 80.00%,70.00% and 55.00%,which was significantly higher than that in normal skin (P<0.05).The positivity rates of p-Akt (Ser473) in SCC,BD and AK were 77.50%,65.00% and 50.00%,while there was no positive staining in normal skin.There was a positive correlation between the expression level of Rictor and p-Akt (Ser473) in SCC,BD and AK (P<0.05).Conclusion:The high expression of Raptor,Rictor and p-Akt (Ser473) may play a key role in the occurrence and development of AK,BD and SCC.