肠道菌群代谢产物IAID对特应性皮炎小鼠模型的作用

目的:确定肠道菌群代谢产物吲哚-3-甲醛(IAID,indole-3-carboxaldehyde)在特应性皮炎(AD)小鼠模型中的作用。方法:将C57小鼠随机分为对照组、模型组和IAID组。小鼠双耳外涂卡泊三醇(MC903)制备AD模型,IAID组给予65μg/kg IAID灌胃,模型组灌等体积DMSO作为对照,对照组不处理。10天后肉眼观察大体炎症情况,千分尺测量小鼠耳廓厚度,ELISA法检测小鼠血清中总IgE水平并通过RT-PCR的方法检测全皮中IL4,IL5,IL6,IL13,IL33,TSLP的表达。结果:IAID组小鼠与模型组相比大体炎症明显减轻,血清总IgE水平明显降低(P0.001),皮损IL13、TSLP的mRNA水平也显著下降(P0.05)。结论:肠道菌群代谢产物IAID可以缓解AD小鼠模型的皮肤炎症。

The impact of gut microbial metabolite on mouse model of atopic dermatitis

Objective: To determine the impact of gut microbial metabolite indole-3-carboxaldehyde ( IAID) on mouse model of atopic dermatitis ( AD) . Methods:C57 mice were divided into 3 groups:control group, model group and IAID group. Vitamin D3 analog MC903 was applied on on mice ear to establish an AD-like model. The mice in IAID group were gavaged with 65μg/kg IAID, and the same amount of DMSO as a vehicle control through administration by gavaging. At day 10, The mice ears were observed for any inflamma￣tory sign and the thickness of the ears were measured. The RT-PCR technique was used to detect the expres￣sion of IL4, IL5, IL6, IL13, IL33, TSLP in whole skin and ELISA technique was used to detect the level of total IgE. Results: Compared to model group, we observed a obvious alleviation in IAID group with a de￣creased level of total IgE (P<0.001) and IL13, TSLP (P<0.05). Conclusion: Gut microbial metabolite IAID can relieve the severity of AD-like inflammation in the mice model.