A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides |
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| Authors: | Akihiko Okamura Shin Yazawa Toyo Nishimura Susumu Tanaka Izumi Takai Shigeharu Kudo Takayuki Asao Hiroyuki Kuwano Khushi L Matta Suguru Akamatsu Naohisa Kochibe |
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| Institution: | (1) First Department of Surgery, Gunma University School of Medicine, Maebashi;(2) Japan Immunoresearch Laboratories, Takasaki, Japan;(3) First Department of Anatomy, Gunma University School of Medicine, Maebashi;(4) Department of Gynecologic Oncology, Roswell Park Memorial Cancer Institute, Buffalo, New York, USA;(5) Diagnostic Division, Otsuka Pharmaceutical Co. Ltd, Tokushima;(6) Department of Biology, Faculty of Education, Gunma University, Maebashi, Japan |
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| Abstract: | A new ex vivo method for assaying adhesion of cancer cells to the greater omentum has been developed using mouse greater omentum and 3H]labelled human gastric and mouse colorectal cancer cells. Since the adhesion rates were found to increase up to 18 h and labelled cells seemed to be stable during the period, the present method could be useful for investigating adhesion of cancer cells to the greater omentum, which must occur at the first step of the peritoneal dissemination. The adhesion of cancer cells to the greater omentum was inhibited by a series of chemically synthesized oligosaccharides and Galβ1,33OMeGalβ1,4GlcNAcβ1,6]αBn was found to be the best inhibitor. The anti-tumor effect of this novel tetrasaccharide in vivo was shown in preliminary experiments using Balb/c mice and colon26 cells. |
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| Keywords: | anti-adhesion therapy cell adhesion chemically synthesized oligosaccharide fucosyltransferase greater omentum peritoneal dissemination |
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