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异种黑色素细胞疫苗诱导小鼠抗恶性黑色素瘤免疫反应
引用本文:罗锋,魏于全,阚兵,杨莉,田玲,卢铀,彭枫,姜愚,刘继彦,赵霞,邹立群,雷松,毛咏秋. 异种黑色素细胞疫苗诱导小鼠抗恶性黑色素瘤免疫反应[J]. 中华肿瘤杂志, 2001, 23(2): 118-121
作者姓名:罗锋  魏于全  阚兵  杨莉  田玲  卢铀  彭枫  姜愚  刘继彦  赵霞  邹立群  雷松  毛咏秋
作者单位:1. 华西医科大学第一附属医院肿瘤生物治疗研究中心肿瘤中心
2. 华西医科大学第二附属医院妇产科
3. 华西医科大学第一附属医院肿瘤生物治疗研究中心
基金项目:国家自然科学基金杰出青年基金资助项目(39725031);国家973项目分题基金资助项目(G1998051214)
摘    要:目的:研究异种黑色素细胞疫苗对小鼠恶性黑色素瘤生长的抑制新作用。方法制轩异种黑色素细胞疫苗,皮皮预防免疫小鼠后再接种恶性黑色素瘤,并以该疫苗皮下注射治疗恶性黑色素瘤鼠,随后测量肿瘤大小,采用^51Cr释放法,体外分析小鼠脾细胞的细胞毒性T细胞(CTL)活性,以间接ELISA法检测小鼠血清抗肿瘤抗 。结果预防免疫使90%小鼠恶性黑色素瘤生长受到抑制,疫苗治疗使50%小鼠肿瘤生长受到抑制,免疫组鼠较对照组CTL杀伤活性在20:1、10:1、5:1不同效靶比时,分别增高34.0%、24.1%及13.9%(P<0.05),体内出现抗恶性黑色素瘤抗体。结论:异种黑色素细胞疫羁可诱导小鼠抗恶性黑色素瘤特异性免疫反,抑制肿瘤生长。

关 键 词:黑色素瘤 免疫 异种黑色素细胞疫苗
修稿时间:2000-03-15

Anti-tumor immune response against mouse melanoma to xenogeneic vaccination
LUO Feng,WEI Yuquan,KAN Bing,et al.. Anti-tumor immune response against mouse melanoma to xenogeneic vaccination[J]. Chinese Journal of Oncology, 2001, 23(2): 118-121
Authors:LUO Feng  WEI Yuquan  KAN Bing  et al.
Affiliation:Center of Cancer Biotherapy, First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041, China.
Abstract:OBJECTIVE: To study the inhibition of melanoma growth in mice by vaccination with xenogeneic melanocytes. METHODS: Xenogeneic vaccine was prepared from pig eye melanocytes. It was used before or after B16 melanoma challenge in C57 mice. The size of tumor was monitored. Cytotoxic T lymphocyte (CTL) activity of mouse spleen cells was measured by 51Cr release assay. Antibody response against pig melanocytes and B16 melanoma cells were detected by indirect ELISA. RESULTS: Preventive vaccination resulted in inhibition of tumor growth in 90% of the immunized mice, while therapeutic vaccination inhibited tumor growth in 50% of the treated mice. Specific CTL activity and antibodies in the immunized mice were detected. CONCLUSION: Anti-tumor immune response capable of inhibiting melanoma growth can be induced by xenogeneic melanocyte vaccination.
Keywords:Melanoma/immunology  Xenogeneic melanocyte vaccine
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