Induction of apoptosis by chelerythrine chloride through mitochondrial pathway and Bcl-2 family proteins in human hepatoma SMMC-7721 Cell |
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| Authors: | Zheng-fu Zhang Ying Guo Jian-bin Zhang Xiong-hui Wei |
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| Institution: | (1) International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai, China;(2) Department of Surgery, Eastern Hepatobiliary Surgery Hospital, Shanghai, China;(3) State Key Laboratory for Oncogenes and Related Genes, Cancer Institute of Ren Ji Hospital, Shanghai Jiao Tong University, Shanghai, China |
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| Abstract: | The objective of this study was to evaluate the antitumor activity of chelerythrine chloride (CHE) and investigate its potential
apoptotic induction mechanism in SMMC-7721 cells. Our results suggested that the proliferation of SMMC-7721 cells was inhibited
by CHE in a time and dose dependent manner, with a significant accumulation in S phase, and the cells exhibited typical apoptotic
features. Moreover, CHE remarkably induced apoptosis by disruption of the mitochondrial membrane potential, release of Cyt-c,
activation of caspase-3, and cleavage of poly-ADP-ribose polymerase in a dose dependent manner. Furthermore, the expression
of Bcl-xl was downregulated while Bax and Bid expression was upregulated, and no variation was found for Bcl-2. These results
indicated that CHE may play an important role in suppression of tumor growth by inducing apoptosis in human hepatoma cells
via the activation of a mitochondrial pathway and regulating the expression of Bcl-2 family proteins. |
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