山东省HIV-1流行株多基因区亚型分析

目的 了解流行于山东省境内HIV-1毒株的亚型，尤其是流行重组型毒株的种类及变异情况，分析其来源并推测其流行趋势。方法采集93份HIV-1感染者的外周静脉防凝血，提取前病毒DNA进行体外扩增，获得包膜蛋白（env）、核心蛋白（gag）、tat区基因的核酸片段，并对各基因区核苷酸进行测定和分析。结果发现在所有目标人群中共存在B、B，、C3种亚型以及CRF07-BC、CRF08-BC、CRF01-AG和CRF01-AE4种重组毒株，其中B’亚型数量最大71株（占77．2％），CRF01-AE9株（占9．8％），CRF07-BC、CRFOB-BC各3株（均占3．3oA），B、C和CRF02-AG各2株（占2．2％）。3个基因区env区变异最大，gag和tat相对保守。3种优势亚型中，A／E变异最大，B’次之，B’／C最保守。结论山东省HIV-1流行毒株亚型众多。各优势毒株发生了不同程度的变异，在山东省有流行加快的趋势，应加强防控。

Subtyping analysis of HIV-1 strains in Shandong province

Objective To understand the distribution of HIV-1 strain subtypes and their variation in Shandong province, analyse their source and predict their epidemic trend. Methods 93 DNA fragments of HIV-1 env, gag, tat gene were amplified by nested PCR from those infected with HIV-1, in 2002-2003. Their C2-V3, P17/P24, 1st exon of tat and adjacent region were sequenced. Results Sequence analysis showed that there were 7 HIV-1 or circulating recombinant forms(CRFs), B'(n=71), CRF01-AE(n=9),CRF07-BC(n=3), CRF0B-BC(n=3), B(n=2),C(n=2), CRF02-AG(n=2). B' strains' variation were much more than CRF01-AE and B'/C.Conclusion There were many kinds of subtype and CRFs, and their genomes generated obvious variation. Therfore, the quickening trend of HIV-1 infection urged the strengthening of HIV control in Shandong province.