妊娠滋养细胞肿瘤患者及治愈后再生育子女外周血染色体脆性部位的研究

对16例未经化疗的GTT患者、15例正常妇女、11例GTT痊愈妇女、14例GTT痊愈妇女再生育子女及12例正常儿童的外周血染色体畸变率及脆性部位进行了观察。结果表明,GTT患者的染色体畸变率、脆性部位表达均明显高于对照组(P<0.01)。化疗痊愈妇女及其子女的染色体畸变和脆性部位表达均与对照组无区别。

Studies on Chromosomal Fragile Site in Peripheral Lymphocytes of Patients with Chemotherapeutically Cured GTT and of their Offsprings

Chromosomal aberrations and fragile sites were studied in cultured peripheral blood lymphocytes of 68 subjects, including 15 normal women, 16 non-treated patients with GTT, 11 women with chemotherapeutically cured GTT, 12 normal children and 14 offsprings of the chemotherapeutically cured women. The results showed that the chromosomal aberration rate of the patients(19.63%) was significantly higher than that of normal women(7.0%). In situ G-banding analysis revealed that 241 breaks and gaps were consistent with the fragile sites. There were 45 breaks and gaps at the sites where some oncogenes locate. Comparing the chemotherapeutically cured patients with the control, and the offsprings of the chemotherapeutically cured patients with normal children, we found no significant difference in their chromosomal aberration rates. The results suggested that chromosomal aberration rate may be decreased or disappeared after the patients were cured by chemotherapy and had stopped chemotherapy for above two years.