| 受维甲酸调控基因的筛选及其诱导分化 |
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| 引用本文： | 蔡绍京,张吉旺,张庆华,沈宇,陈赛娟. 受维甲酸调控基因的筛选及其诱导分化[J]. 江苏医药, 2006, 32(1): 43-44 |
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| 作者姓名： | 蔡绍京 张吉旺 张庆华 沈宇 陈赛娟 |
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| 作者单位： | 221002,徐州医学院生物学教研室;上海第二医科大学附属瑞金医院 |
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| 基金项目： | 江苏省教育厅自然科学基金 |
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| 摘 要： | 目的 筛选受维甲酸调控的新基因片段，探讨这些基因诱导分化作用的机制。方法 经细胞培养，构建人急性早幼粒细胞性白血病细胞的减数文库，应用半定量RT-PCR技术对减数文库的前向文库中53个新基因片段进行筛选。结果 24个新基因片段受维甲酸调控，其中大多数呈上调趋势，少数表现为先下调后上调。结论 维甲酸诱导的基因表达效应具有明显的时相特征。
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| 关 键 词： | 维甲酸 急性早幼粒白血病 基因调控 |
| 收稿时间： | 2004-09-16 |
| 修稿时间： | 2004-09-16 |
Screening and differentiation-inducing effect of genes modulated by retinoic acid |
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| CAI Shaojing, ZIIANG Jiwang , ZHANG Qinghua ,et al.. Screening and differentiation-inducing effect of genes modulated by retinoic acid[J]. Jiangsu Medical Journal, 2006, 32(1): 43-44 |
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| Authors: | CAI Shaojing ZIIANG Jiwang ZHANG Qinghua et al. |
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| Affiliation: | Department of Biology, Xuzhou Medical College, Xuzhou 221002, CHINA |
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| Abstract: | Objective To screen novel gene segments modulated by retinoic acid and study differentiation-inducing effect of the genes.Methods Using cell culture,construction of subtractive(library) for human's acute promyelocytic leukemia cell and semiquantative RT-PCR technique,53 novel gene segments were screened in the forward library of subtractive library.Results Twenty four novel gene segments were modulated by retinoic acid,among which the majority were up-regulated,the(minority) were down-regulated at the beginning,then up-regulated.Conclusion Gene expression(induced) by retinoic acid was regularly changed with phases. |
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| Keywords: | Retinoic aeid Acute promyelocytic leukemia Gene regulation |
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