人脑胶质瘤中PCNA、Ki-67和Mitosin/CENP-F的表达研究

目的 检测PCNA、Ki-67和Mitosin/CENP-F在人脑胶质瘤的表达,探讨它们与胶质瘤恶性生物学行为的关系.方法 应用免疫组化S-P法检测70例不同病理分级、不同组织学分型人脑胶质瘤和24例非肿瘤脑组织中PCNA、Ki-67和Mitosin/CENP-F的表达.结果 (1)70例脑胶质瘤标本中,PCNA、Ki-67和Mitosin/CENP-F的增殖标记指数(LI)分别为(44.02±29.37)%、(17.01±17.68)%和(36.80±27023)%;对照组24例非肿瘤脑组织中Ki-67不表达,PCNA LI和Mitosin/CENP-F LI为(12.11±19.81)%和(9.06+8.27)%,两组比较具有显著差异(P<0.01).(2)PCNA、Ki-67和Mitosin/CENP-F的表达与胶质瘤患者的年龄、性别无关,与病理分级和组织学分型相关(P<0.05).结论 人脑胶质瘤中PCNA、Ki-67和Mitosin/CENP-F的LI与胶质瘤的病理分级和病理分型密切相关,均可作为判断胶质瘤恶性程度的重要指标.

Expressions of PCNA, Ki-67 and Mitosin/CENP-F proteins in human gliomas

Objective To study the expressions of PCNA, Ki-67 and Mitosin/CENP-F and the relationship with biological features of human gliomas. Methods The Labeling Index (LI) of PCNA, Ki-67 and Mitosin/CENP-F proteins was detected by immunohistochemistry in 70 cases of human glioma of various pathological types and grades and 24 cases of non-tumor brain tissue samples. Results LI values of PCNA, Ki-67 and Mitosin/CENP-F in 70 cases of glioma were (44.02±29.37)%, (17.01±17.68)% and (36.80±27.23)%, respectively. In non-tumor brain tissue samples, the expression of Ki-67 was negative, and LI values of PCNA and Mitosin/CENP-F were (12.11±19.81)% and (9.06±8.27)%. There were statistically significant differences between glioma and non-tumor brain tissue samples (P<0.01). In addition, the correlation between the increasing grade and various histologic types and PCNA, Ki-67 and Mitosin/CENP-F LI values were statistically significant (P<0.05). However, no correlation was detected between the age and sex of the patiets and any one of three markers. Conclusion PCNA, Ki-67 and Mitosin/CENP-F, three tumor markers can be used to evaluate the tumor grade and to asses the malignancy in human gliomas.