| 家蝇幼虫血淋巴纯化蛋白对Hela肿瘤细胞抑制作用的形态学观察 |
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| 引用本文： | 魏洪,吴建伟,王硕石,王江兰,国果,付萍.家蝇幼虫血淋巴纯化蛋白对Hela肿瘤细胞抑制作用的形态学观察[J].中国媒介生物学及控制杂志,2011,22(6):525-528. |
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| 作者姓名： | 魏洪 吴建伟 王硕石 王江兰 国果 付萍 |
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| 作者单位： | 1. 遵义医学院微生物学教研室,贵州遵义,563003
2. 贵阳医学院寄生虫学教研室,贵州贵阳,550004 |
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| 基金项目： | 国家自然科学基金(30960343)~~ |
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| 摘 要： | 目的 研究家蝇幼虫血淋巴纯化蛋白(anticancer protein from Musca domestica larva hemolymph,MAC-1)对体外培养的人官颈癌Hela细胞抑制作用引起的形态学改变.方法 应用四甲基偶氮噻唑蓝(MTT)法、光学显微镜、HE和吖啶橙(AO)染色、透射电镜(SEM)的方法检测MAC-1对体外培养的人宫颈癌Hela细胞的抑制作用及形态学改变.结果 MAC-1对人宫颈癌Hela细胞株的增殖抑制作用呈剂量依赖性,同时呈时间依赖性.MAC-1 12.5 μg/ml作用12h后在倒置显微镜、HE及AO染色和透射电镜均观察到典型细胞凋亡形态变化.结论 MAC-1对人宫颈癌Hela细胞株具有抑制增殖及诱导凋亡作用.
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| 关 键 词： | 家蝇幼虫 血淋巴 蛋白 人宫颈癌Hela细胞株 细胞凋亡 |
Morphological observation of Hela human cervical carcinoma cells suppressed by the immuned hemolymph of housefly larvae protein |
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| WEI Hong
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WU Jian-wei
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WANG Suo-shi
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WANG Jiang-lan
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GUO Guo
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FU Ping.Morphological observation of Hela human cervical carcinoma cells suppressed by the immuned hemolymph of housefly larvae protein[J].Chinese Journal of Vector Biology and Control,2011,22(6):525-528. |
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| Authors: | WEI Hong WU Jian-wei WANG Suo-shi WANG Jiang-lan GUO Guo FU Ping |
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| Institution: | WEI Hong~1,WU Jian-wei~2,WANG Suo-shi~2,WANG Jiang-lan~2,GUO Guo~2,FU Ping~2 1 Department of Microbiology,Zunyi Medical College,Zunyi 563003,Guizhou Province,China,2 Department of Parasitology,Guiyang Medical College,Guiyang 550004 |
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| Abstract: | Objective To observe the morphological changes of Hela human cervical carcinoma cells in vitro after treatment with the anticancer protein from Musca domestica larva hemolymph(MAC-1) protein derived from the immuned hemolymph of housefly larvae.Methods The growth inhibition rates and cytotoxic effects of Hela cells were measured with MTT assay.The treated cells were observed under inverted microscope and morphological changes were tested with HE and microscope.The ultrastructure changes were observed under ... |
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| Keywords: | Musca domestica larvae Hemolymph Protein Hela human cervical carcinoma cell Apoptosis |
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