沙尘暴PM2.5对大鼠肺细胞DNA损伤效应

目的探讨沙尘暴细颗粒物（PM205）对大鼠肺细胞的遗传损伤作用。方法采用气管直接注入染毒法，分别给大鼠灌注0，1．5，7．5，37．5mg／kg剂量的颗粒物悬浮液。灌注24h后处死大鼠，采用单细胞凝胶电泳技术（SCGE）检测肺细胞DNA的损伤。结果沙尘暴和正常天气PM2.5均可引起大鼠肺细胞DNA损伤，且随剂量增加而损伤增大。正常天气PM2．5比沙尘暴PM2．5对肺细胞DNA的损伤更大，但差异无统计学意义。结论沙尘暴和正常天气PM2.5均可引起大鼠肺细胞DNA的损伤，由于沙尘暴PM2.5浓度远高于正常天气PM2.5，导致沙尘暴PM2.5对DNA的毒性远大于正常天气PM2．5。

Damaging effects of dust storm fine particles instillation on DNA in lung cells of rats

Objective To investigate damaging effects of dust storm fine particle(PM_(2.5)) on DNA in lung cells of rats.Methods Wistar rats were randomly divided into toxicosis groups and control group by intratracheal instillation.After instilled 24 hours,DNA damage of lung cells of rats was detected with single cell gel electrophoresis technique.Results Both dust storm PM_(2.5) and normal weather PM_(2.5) could lead to DNA damage in a dose-dependent manner.Though the effects(induced) by normal weather PM_(2.5) heavier than dust storm PM_(2.5),there was no significant difference between them.Conclusion Both dust storm PM_(2.5) and normal weather PM_(2.5) could lead to DNA damage in lung cells of rats.However,the dust storm PM_(2.5) whose airborne concentrations were much higher than that of normal weather PM_(2.5),so the dust storm PM_(2.5) should be more harmful.