e抗原阳性慢性乙型肝炎患者血清HBV-DNA与HBsAg、HBeAg的相关性分析

目的 研究e抗原阳性慢性乙型肝炎患者外周血中HBV-DNA载量与乙型肝炎病毒表面抗原（HBsAg）、乙型肝炎病毒e抗原（HBeAg）的相关性,及其在不同性别、年龄群体中的差异.方法 收集319例e抗原阳性慢性乙肝患者血清,采用实时荧光定量PCR法检测HBV-DNA载量,用时间分辨免疫荧光法检测HBsAg和HBeAg的浓度,利用SPSS软件做统计分析.结果 HBV-DNA载量与HBsAg含量有良好的相关性（r=0.514,P〈0.001）;与HBeAg含量有相关（r=0.337,P〈0.001）;女性的HBeAg水平要高于男性患者（P〈0.05）;年龄（31～50）岁组、〉50岁组的HBV-DNA、HBsAg 及HBeAg值皆高于年龄 〈30岁组 （P〈0.001）.结论 e抗原阳性慢性乙型肝炎患者血清中HBV-DNA载量与HBsAg、HBeAg定量水平皆有相关性,其中与HBsAg相关性更佳.

Correlative Analysis on Serum HBV-DNA, HBsAg and HBeAg Levels in Chronic Hepatitis B Patients with e Antigen Positive

Objective To study the relationship among hepatitis B virus DNA(HBV-DNA),HBsAg and HBeAg levels in chronic hepatitis B patients with e antigen positive.Methods HBV-DNA,HBsAg and HBeAg levels of 319 chronic hepatitis B patients with e antigen positive were detected by realtime-PCR,time-resolved fluoroimmunoassay(TrFIA) and auto biochemistry analyzer respectively.The data was analyzed by SPSS software.Results There was highly correlation(r=0.514,P<0.001) between HBV-DNA and HBsAg levels,and there was also some correlation(r=0.337,P<0.001) between HBV-DNA and HBeAg levels.It was found that HBeAg levels in female patients were higher than in male patients(P<0.05);and HBV-DNA,HBsAg,HBeAg levels in middle-aged patients or elderly patients were less than in young patients(P<0.001).Conclusion There was correlation between HBV-DNA and HBsAg levels in chronic hepatitis B patients with e antigen positive.