干燥综合征患者Th17与调节性T细胞免疫失衡的观察

目的 检测干燥综合征（Sjogren＇s syndrome,SS）患者唇腺组织及外周静脉血中Th17细胞和调节性T细胞（Treg）相关细胞因子的表达水平,分析Th17/Treg免疫平衡在SS发病机制中的作用.方法 采用逆转录-聚合酶链反应（RT-PCR）法,检测60例SS（实验组）和30例正常唇腺组织（对照组）中IL-17A、FOXP3mRNA的表达水平;另采用ELISA检测上述两组血清中IL-17、IL-10的含量.结果与对照组相比,SS患者唇腺组织内IL-17AmRNA的表达显著升高（P〈0.05）（SS者唇腺组织中FoxP3mRNA的表达降低（P〈0.05）SS患者血清IL-17水平明显高于对照组（P〈0.05）,SS患者血清IL-10水平明显低于对照组（P〈0.05）.结论 SS患者唇腺组织和外周血中Th17细胞应答增强,而Treg细胞免疫抑制下降;Th17/Treg免疫失衡可能参与SS的发生发展.

The Imbalance of Immunity Between Th17 and CD4+ CD25+ Regulatory T Cell in Patients with Sj(o)gren's Syndrome

Objective To observe the expression of related cytokines of Th17 and CD4+ CD25+ regulatory T cell in the labial gland and peripheral blood from patients with Sjgren’s syndrome and to explore the role of Th17/Treg balance in the pathogenesis of Sjgren’s dysdrome.Methods Labial glands and peripheral blood were collected from 60 patients with Sjgren’s dysdrome and 30 normal subjects without SS as control group.The expression of IL-17A and Foxp3mRNA in labial glands were measured by RT-PCR.The levels of related cytokines IL-17 and IL-10 in peripheral blood were assayed by ELISA.Results Compared with control group,in patients with SS,the expression of IL-17A mRNA in labial glands increased significantly(t=0.5061,P<0.05);the expression of FoxP3mRNA in labial glands decreased apparently in SS patients(t=0.5298,P<0.05);the serum level of IL-17 in patients with SS increased notably(t=5.5840,P<0.05);the serum level of IL-10 decreased obviously in SS patients(t=17.9170,P<0.05).Conclusion The increased response of Th17 cells and diminished immunosuppression of Treg cells are presented both labial glands and peripheral blood in patients with SS.It is suggested that the imbalance of immunity between Th17 and Treg may play an important role in the process of SS.