| 感染鼠肝石蜡切片免疫酶染色法诊断血吸虫病的研究 |
| |
| 引用本文： | 陈淑贞,管晓虹,蔡银龙. 感染鼠肝石蜡切片免疫酶染色法诊断血吸虫病的研究[J]. 南京医科大学学报(自然科学版), 1986, 0(4) |
| |
| 作者姓名： | 陈淑贞 管晓虹 蔡银龙 |
| |
| 作者单位： | 南京医学院寄生虫学研究室，南京医学院寄生虫学研究室，南京医学院寄生虫学研究室 |
| |
| 摘 要： | 本研究应用福尔马林固定的感染鼠肝石蜡切片免疫酶染色法。检测109例血吸虫病病人血清,阳性率达95.41%;141例正常人血清的假阳性率为1.42%,表明该法有较高的敏感性及特异性,具有一定的诊断价值。实验中并对所应用的底物及有关步骤作了比较及改进。
|
| 关 键 词： | 血吸虫病 日本血吸虫 肝疾病 寄生虫病 肝 血清诊断 免疫酶技术 |
THE STUDY OF IMMUNOENZYMATIC TECHNIQUE FOR THE DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA USING LIVER SECTIONS OF INFECTED MICE |
| |
| Chen Shuzhen,et al.. THE STUDY OF IMMUNOENZYMATIC TECHNIQUE FOR THE DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA USING LIVER SECTIONS OF INFECTED MICE[J]. Acta Universitatis Medicinalis Nanjing, 1986, 0(4) |
| |
| Authors: | Chen Shuzhen et al. |
| |
| Abstract: | This paper concerns the application of an immunoenzymatic techniqueperformed on formalin-fixed and paraffin-embedded liver-egg sections of mice infected with Schistosoma japonicum for the diagnosis of schistosomlasis.Antibodies wete detected by the technique in 109 serum samples from patients with schistosomiasis,The positive rate was as high as 95.41% and the false positive rate of 141 uninfected control sera was 1.42%.It was shown that the method described is sensitive and specific enough to meet the requirement of the diagnosis of the disease.In the experiment,it has been made to improve the stain procedure of samples which is described by Mesulam(1976)and compare the resultant color developed by these two substrates. |
| |
| Keywords: | Schistosomiasis Schistosomiasis Japonicum Liver diseases parasitic Liver Serodiagnosis Immunonzyme technics |
| 本文献已被 CNKI 等数据库收录！ |
