| 奥沙利铂长循环脂质体对人结直肠癌SW480细胞凋亡影响 |
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| 引用本文： | 杨闯,刘海忠,傅仲学. 奥沙利铂长循环脂质体对人结直肠癌SW480细胞凋亡影响[J]. 第三军医大学学报, 2011, 33(4): 368-371 |
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| 作者姓名： | 杨闯 刘海忠 傅仲学 |
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| 作者单位： | 重庆医科大学附属第一医院普通外科;绵阳市第三人民医院肝胆外科;重庆医科大学附属第二医院妇产科; |
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| 基金项目： | 重庆市卫生局科研基金(2009-2-12)~~ |
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| 摘 要： | 目的探讨奥沙利铂长循环脂质体对人结直肠癌SW480细胞的凋亡作用。方法采用荧光标记的长循环脂质体,奥沙利铂长循环脂质体和游离奥沙利铂分别处理人结直肠癌SW480细胞,流式细胞仪、荧光显微镜检测细胞对脂质体的摄取;MTT分析载药脂质体对细胞增殖影响;透射电镜观察细胞超微结构变化;流式细胞仪及TUNEL检测细胞凋亡;Western blot分析actived-caspase-3(P17)的表达。结果脂质体被细胞摄取,2 h的荧光强度为(197.72±8.92),24 h的荧光强度为(641.74±44.46);2.6 mmol/L的奥沙利铂长循环脂质体(含28 mg/L奥沙利铂)对细胞的活性影响及凋亡诱导作用大于28 mg/L游离奥沙利铂(P<0.01);actived-caspase-3蛋白的表达随细胞凋亡增加而上调(P<0.01)。结论奥沙利铂长循环脂质体对人结直肠癌SW480细胞有显著凋亡诱导作用。
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| 关 键 词： | 脂质体 奥沙利铂 结直肠肿瘤 凋亡 |
Oxaliplatin long-circulating liposomes improves apoptosis of human colorectal cancer SW480 cells |
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| Yang Chuang,Liu Haizhong,Fu Zhongxue. Oxaliplatin long-circulating liposomes improves apoptosis of human colorectal cancer SW480 cells[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(4): 368-371 |
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| Authors: | Yang Chuang Liu Haizhong Fu Zhongxue |
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| Affiliation: | Yang Chuang1,2,Liu Haizhong3,Fu Zhongxue1(1Department of General Surgery,First Affiliated Hospital,Chongqing Medical University,Chongqing,400016,2Department of Hepatobiliary Surgery,Mianyang Third People's Hospital,Mianyang,Sichuan Province,621000,3Department of Gynecology and Obstetrics,Second Affiliated Hospital,400010,China) |
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| Abstract: | Objective To investigate the effect of oxaliplatin long-circulating liposomes(PEG-liposomal L-oHP) on the apoptosis of human colorectal cancer SW480 cells.Methods Colorectal cancer SW480 cells were treated with fluorescent labeled PEG-liposomes,PEG-liposomal L-oHP and free L-oHP,respectively.Cellular uptake of fluorescent-labeled PEG-liposomes were detected by flow cyometry(FCM) and fluorscence microscopy.The cells proliferation were analyzed by MTT assay.Cellular ultrastructure was observed with transmissi... |
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| Keywords: | liposomes oxaliplatin colorectal neoplams apoptosis |
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