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携带siANGPTL4重组腺病毒的构建及其对MG63增殖的抑制作用
引用本文:洪思琦,毕杨,郭振华,陈镜宇,李映良. 携带siANGPTL4重组腺病毒的构建及其对MG63增殖的抑制作用[J]. 第三军医大学学报, 2011, 33(1): 28-32
作者姓名:洪思琦  毕杨  郭振华  陈镜宇  李映良
作者单位:重庆医科大学附属儿童医院神经内科;重庆医科大学儿童发育疾病研究省部共建教育部重点实验室;
基金项目:重庆市科委自然科学基金(CSTC2010BB5094,CSTC2007BB5295)~~
摘    要:目的 应用AdEasy腺病毒改良载体系统构建携带siANGPTL4基因的重组腺病毒,进一步研究ANGPTL4基因对骨肉瘤细胞株MG63增殖的影响.方法 将设计的siRNA靶点寡聚核苷酸克隆到pSES-HUS载体构建重组质粒pSES-siANGPTL4,在BJ5183大肠杆菌内与pAdEasy1骨架质粒完成同源重组;经...

关 键 词:腺病毒  血管生成素样蛋白4  转染  骨肉瘤  红色荧光蛋白

Construction of recombinant adenovirus with siANGPTL4 gene and its inhibitive effect on MG63 cell proliferation
Hong Siqi,Bi Yang,Guo Zhenhua,Chen Jingyu,Li Yingliang. Construction of recombinant adenovirus with siANGPTL4 gene and its inhibitive effect on MG63 cell proliferation[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(1): 28-32
Authors:Hong Siqi  Bi Yang  Guo Zhenhua  Chen Jingyu  Li Yingliang
Affiliation:Hong Siqi1,2,Bi Yang2,Guo Zhenhua2,Chen Jingyu2,Li Yingliang2(1Department of Neurology,2Key Laboratory of Developmental Diseases in Childhood Chongqing and Ministry of Education,Children's Hospital,Chongqing Medical University,Chongqing,400014,China)
Abstract:Objective To construct recombinant adenovirus with si-angiopoietin-like 4(siANGPTL4) gene using modified AdEasy system,and to investigate the effect of ANGPTL4 gene on the proliferation of osteosarcoma cell line MG63.Methods The designed siRNA oligonucleotide fragments of ANGPTL4 gene were cloned into shuttle plasmid pSES-HUS to construct recombinant plasmid pSES-siANGPTL4,and homologous recombination was completed between pSES-siANGPTL4 and backbone plasmid pAdEasy1 in E.coli BJ5183 to construct recombinan...
Keywords:adenovirus  angiopoietin-like 4  transfection  osteosarcoma  red fluore scent protein RFP  
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