| hVEGF121基因真核表达载体的构建及其在神经干细胞中的表达 |
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| 引用本文： | 张猛,邓娟,李静,王延江,周华东.hVEGF121基因真核表达载体的构建及其在神经干细胞中的表达[J].第三军医大学学报,2011,33(1):36-39. |
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| 作者姓名： | 张猛 邓娟 李静 王延江 周华东 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所神经内科; |
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| 基金项目： | 创伤、烧伤与复合伤国家重点实验室开放基金(SKLKF201018)~~ |
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| 摘 要： | 目的 构建真核表达载体pEGFP-N1/hVEGF121,并转染神经干细胞(neural stem cells,NSCs),以探究人血管内皮生长因子(vascular endothelial growth factor,hVEGF121)基因导入NSCs的表达变化.方法 分离培养胎鼠NSCs,并行nestin、GFAP...
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| 关 键 词： | 人血管内皮生长因子基因 真核表达载体 神经干细胞 绿色荧光蛋白 |
Construction of eukaryotic expression vector of human vascular endothelial growth factor for transfecting neural stem cells |
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| Zhang Meng,Deng Juan,Li Jing,Wang Yanjiang,Zhou Huadong.Construction of eukaryotic expression vector of human vascular endothelial growth factor for transfecting neural stem cells[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(1):36-39. |
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| Authors: | Zhang Meng Deng Juan Li Jing Wang Yanjiang Zhou Huadong |
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| Institution: | Zhang Meng,Deng Juan,Li Jing,Wang Yanjiang,Zhou Huadong(Department of Neurology,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing,400042,China) |
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| Abstract: | Objective To construct eukaryotic expression vector pEGFP-N1/hVEGF121 for transfecting neural stem cells(NSCs),and to investigate the expression of human vascular endothelial growth factor(hVEGF121) in transfected NSCs.Methods NSCs were isolated from fetal mice and cultured,and then identified by immunohistochemical staining of nestin and GFAP.Full-length hVEGF121 cDNA was amplified by PCR using hVEGF121 plasmid as template.The amplified cDNA fragments were cloned into plasmid vector pMD18-T,and then subclo... |
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| Keywords: | vascular endothelial growth factor eukaryotic expression vector neural stem cells green fluorescent protein |
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