| 应用骨髓干细胞和自制胶原-壳聚糖构建组织工程心瓣膜 |
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| 引用本文： | 付建华,赵曼,杜桂英,杨达宽,黄云超. 应用骨髓干细胞和自制胶原-壳聚糖构建组织工程心瓣膜[J]. 第三军医大学学报, 2011, 33(2): 152-155 |
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| 作者姓名： | 付建华 赵曼 杜桂英 杨达宽 黄云超 |
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| 作者单位： | 聊城市人民医院胸心外科;昆明医学院第二附属医院胸心外科;聊城市人民医院信息科;聊城市疾控中心艾防所;昆明医学院第三附属医院胸心外科; |
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| 摘 要： | 目的探讨应用骨髓基质干细胞(bone mesenchymal stem cells,BMSCs)诱导分化的内皮细胞为种子细胞,以胶原-壳聚糖复合物为支架材料,运用组织工程学的原理和方法体外构建组织工程心脏瓣膜(tissue engineering heart valve,TEHV)的可行性。方法兔主动脉壁体外分离、培养、传代平滑肌细胞、成纤维细胞,将培养的成纤维细胞、平滑肌细胞与兔骨髓基质干细胞诱导分化的内皮细胞按顺序种植在预湿处理的胶原-壳聚糖复合支架上,通过组织学及倒置显微镜、扫描电镜观察内皮细胞、成纤维细胞、平滑肌细胞在支架上生长、增殖和基质分泌情况。结果 HE染色示内皮细胞能够在胶原-壳聚糖复合支架黏附、伸展、增殖,在TEHV表面长成连续的细胞层;扫描电子显微镜示TEHV表面光滑,细胞生长良好,细胞层完整,且瓣膜表面的内皮细胞具有合成和分泌前列环素(PGI2)的功能。结论以兔主动脉壁体外分离、培养、传代的平滑肌细胞、成纤维细胞和BMSCs诱导分化的内皮细胞为种子细胞,依次种植在胶原-壳聚糖复合支架上可以构建TEHV,且内皮细胞能够分泌PGI2。
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| 关 键 词： | 组织工程心脏瓣膜 瓣膜构建 骨髓基质干细胞 诱导分化 内皮细胞 |
Construction of tissue engineering heart valve with rabbit bone mesenchymal stem cells and self-made collage-chitosan complex scaffold |
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| Fu Jianhua,Zhao Man,Du Guiying,Yang Dakuan,Huang Yunchao. Construction of tissue engineering heart valve with rabbit bone mesenchymal stem cells and self-made collage-chitosan complex scaffold[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(2): 152-155 |
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| Authors: | Fu Jianhua Zhao Man Du Guiying Yang Dakuan Huang Yunchao |
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| Affiliation: | Fu Jianhua,Zhao Man,Du Guiying,Yang Dakuan,Huang Yunchao(Department of Cardiac , Thoracic Surgery,Department of Information,Liaocheng People’s Hospital,Liaocheng,Sh,ong Province,Department of Cardiac , Thoracic Surgery,Second Affiliated Hospital,Department of Cardiac , Thoracic Surgery,Third Affiliated Hospital,Kunming Medical College,Kunming,Yunnan Province,Institute of AIDS Prevention , Treatment,Liaocheng Center for Disease Control , Prevention,Sh,ong Pro... |
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| Abstract: | Objective To explore the feasibility of constructing a tissue engineering heart valve(TEHV) using endothelial cells induced and differentiated from(bone mesenchymal stem cells,BMSCs) as seeded cells and collagen-chitosan complex as scaffold material.Methods Smooth muscle cell and fibroblasts were isolated and cultured from rabbit aortic wall and Fibroblast,smooth muscle cells and endothelial cell induced and differentiated from BMSCs were seeded in pre-moisture collagen-chitosan complex scaffold in turn.The... |
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| Keywords: | tissue engineering heart valve valve construction bone mesenchymal stem cells induce and differentiate endothelial cells |
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