| 丝氨酸-苏氨酸激酶受体相关蛋白在健康人和系统性红斑狼疮患者外周血单个核细胞中的差异表达 |
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| 引用本文： | 王林纤,戴勇,彭武建,孙保东,齐素文,涂植光. 丝氨酸-苏氨酸激酶受体相关蛋白在健康人和系统性红斑狼疮患者外周血单个核细胞中的差异表达[J]. 第三军医大学学报, 2011, 33(2): 148-151 |
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| 作者姓名： | 王林纤 戴勇 彭武建 孙保东 齐素文 涂植光 |
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| 作者单位： | 重庆医科大学临床检验诊断学教育部重点实验室;广东省深圳市人民医院暨南大学第二临床医学院临床医学研究中心; |
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| 基金项目： | 深圳市科技计划重点项目(201001001)~~ |
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| 摘 要： | 目的分析丝氨酸-苏氨酸激酶受体相关蛋白在系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血单个核细胞中的表达水平,探讨该蛋白检测在系统性红斑狼疮病情判断中的可能应用。方法实验分为3组:SLE稳定组、SLE活动组和健康对照组,每组6例,分别收集其外周血单个核细胞,用相对和绝对定量的等量异位标签串联质谱技术对丝氨酸-苏氨酸激酶受体相关蛋白进行鉴定和相对定量分析,用Western blot技术对该蛋白的表达水平进行较大样本的独立验证。结果基于质谱分析的蛋白组学结果表明,丝氨酸-苏氨酸激酶受体相关蛋白在系统性红斑狼疮活动期患者外周血单个核细胞中表达显著下调(与正常人的相对比值为0.2906),且该蛋白的低表达为Western blot实验进一步证实。临床资料分析表明,该蛋白的表达水平与系统性红斑狼疮疾病活动指数呈负相关(r=-0.607,P<0.05)。结论丝氨酸-苏氨酸激酶受体相关蛋白可能参与SLE的发病,该蛋白有望作为系统性红斑狼疮的活动阶段特异性标志物。
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| 关 键 词： | 丝氨酸-苏氨酸激酶受体相关蛋白 系统性红斑狼疮 外周血单个核细胞 表达 |
Differential expression of serine-threonine kinase receptor-associated protein in healthy subjects and patients with systemic lupus erythematosus |
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| Wang Linqian,Dai Yong,Peng Wujian,Sun Baodong,Qi Suwen,Tu Zhiguang. Differential expression of serine-threonine kinase receptor-associated protein in healthy subjects and patients with systemic lupus erythematosus[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(2): 148-151 |
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| Authors: | Wang Linqian Dai Yong Peng Wujian Sun Baodong Qi Suwen Tu Zhiguang |
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| Affiliation: | 1 (1Key Laboratory of Laboratory Medical Diagnostics Cofounded by Chongqing and Ministry of Education,Chongqing Medical University,Chongqing,400016;2 Research Center of Clinical Medicine,Second Clinical Medical College,Jinan University,Shenzhen People’s Hospital,Shenzhen,Guangdong Province,518020,China) |
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| Abstract: | Objective To analyze the expression level of serine-threonine kinase receptor-associated protein(STRAP) in peripheral blood mononuclear cells of patients with systemic lupus erythematosus(SLE) and to investigate the possible application of STRAP test in evaluation of SLE.Methods Peripheral blood mononuclear cells were collected from 6 stable and 6 active SLE patients and 6 healthy controls,respectively.STRAP was first identified and quantified with isobaric tagging for relative and absolute protein quantifi... |
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| Keywords: | serine-threonine kinase receptor-associated protein systemic lupus erythematosus peripheral blood mononuclear cells expression |
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