甘氨双唑钠修饰纳米金对肺腺癌细胞A549的放射增敏研究

目的 探讨甘氨双唑钠修饰纳米金制备及放射增敏效果。方法 把甘氨双唑钠修饰到已连接聚乙二醇的纳米金上,纳米金粒径18 nm。利用扫描电镜观察肺腺癌(A549)细胞吞噬甘氨双唑钠-纳米金的现象。将培养的肺腺癌细胞分为甘氨双唑钠组、纳米金组、甘氨双唑钠-纳米金组、对照组(不加药组)。用四甲基偶氮唑盐比色法和克隆形成实验对肺腺癌(A549)细胞进行放射增敏的研究。结果 甘氨双唑钠-纳米金能进入细胞质和细胞核;浓度为0.003 mg/ml纳米金和甘氨双唑钠-纳米金没有明显的细胞毒性;甘氨双唑钠-纳米金组相对于甘氨双唑钠组、纳米金组、对照组,D₀、D_q出现了下调;接受2、4、6和8 Gy剂量照射后,甘氨双唑钠-纳米金组肺腺癌细胞的存活率与其他3组相比,均明显下降(F=4.8、14.5、5.7、7.6,P<0.05)。结论 甘氨双唑钠修饰的纳米金能增加肺腺癌细胞的辐射敏感性。

Radiosensitization effect of gold nanoparticles modified by sodium glycididazole on lung adenocarcinoma cell A549

Objective To study the radiosensitization effect of gold nanoparticles modified by sodium glycididazole. Methods The sodium glycididazole was connected to gold nanoparticle, in dimension of about 18 nm, that had been modified with polyethylene glycol. The nanoparticle-swallowing efficiency of lung adenocarcinoma A549 cells was observed by a scanning electron microscope. Cells were divided into four groups: sodium glycididazole group, gold nanoparticles group, sodium glycididazole-gold nanoparticles group, and no drug control group. The radiosensitivity was detected by MTT and colony formation assays. Results Sodium glycididazole-gold nanoparticles could enter the cell cytoplasm and nucleus. The concentration of 0.003 mg/ml gold nanoparticles and sodium glycididazole-gold nanoparticles had no obvious cytotoxic effect. After irradiation of 2, 4, 6, 8 Gy, the cell survival of the sodium glycididazole-gold nanoparticle group was significantly lower than that of the other three groups (F=4.8, 14.5, 5.7, 7.6,P<0.05) and the D₀ and D_q values of the sodium glycididazole-gold nanoparticle group were significantly lower than those of other three groups. Conclusion Sodium glycididazole-gold nanoparticles can enhance the radiosensitivity of lung adenocarcinoma cells.