Bcl-2基因及其家族Bax,Bcl-Xl和Fas/Apo-l,P16的检测在急性白血病中的临床意义

目的：为了解白血病凋亡正、负反馈网络中的问题与临床的关系，研究了抑制凋亡的基因Ｂｃｌ２及其家族Ｂａｘ，ＢｃｌＸｌ以及诱导凋亡的基因Ｆａｓ／Ａｐｏ１，Ｐ１６。方法：采用细胞免疫组化，ＷｅｓｔｅｒｎＢｌｏｔ，以及ＮｏｒｔｈｅｒｎＢｌｏｔ的方法。结果：发现在ＡＭＬ组和ＡＬＬ组，Ｂｃｌ２抗原表达均明显高于正常（Ｐ＜００１），回顾性分析ＡＭＬＣＲ组其明显低于ＮＲ组（Ｐ＜００１）。蛋白印迹ＣＲ组Ｂｃｌ２表达虽然降低，但和ＮＲ组比较无统计学意义（Ｐ＞００５）。Ｂｃｌ２ｍＲＮＡ的表达ＣＲ组明显低于ＮＲ组（Ｐ＜０．０１）。Ｂａｘ的表达在免疫组化中白血病明显低于正常（Ｐ＜００１），但在ＣＲ组与ＮＲ组，免疫组化，蛋白印迹，ＢａｘｍＲＮＡ的表达，均无差异（Ｐ＞００５）。但ＢｃｌＸｌｍＲＮＡ的表达两组间有明显差异（Ｐ＜００１）。Ｆａｓ／Ａｐｏ１的表达为白血病组低于正常（Ｐ＜００１），但在ＣＲ与ＮＲ组中，免疫组化与蛋白印迹分析均无统计差异（Ｐ＞００５）。Ｐ１６的表达为白血病组低于正常组（Ｐ＜００１），但在ＣＲ组与ＮＲ组中无统计学意义（Ｐ＞００５）。结论：Ｂｃｌ２抗原以及Ｂｃｌ２ｍＲＮＡ，Ｂｃｌ

Study on Bcl-2 gene and its family genes Bax,Bcl Xl as well as Fas/Apo 1,P16 genes and their clinical significance in Acute leukemia

Purpose:To understand the positive and negative regulation of apoptosis of leukmic cells and its relationship with clinic,the apoptosis suppressing genes,namely Bcl 2 and its family genes Bax,Bcl Xl and inducing genes Fas/Apo 1 and P16 were studied.Methods:The techniques of cytoimmune histiochemical stains,Westem blot and Northem blot were used.Restults:It was found that the expression of antigens of Bcl 2 in AML and ALL was higher than that in normal(P<0 01).At the same time,Bcl 2 was obviously lower expressed in CR group than that in NR group in retrospective analysis(P<0 01).Though Bcl 2 was low expressed in CR with Westen blot,there was no statistical significance(P>0 05).In CR group the expression of Bcl 2 mRNA was obviously lower than that of NR one(P<0 01). Even though in leukmia expression of Bax by cytoimmune histiochemical stain was also lower than that in normal,there was no difference between CR and NR with cytoimmune histiochemical, Western blot and Northern blot(P>0 05). There was difference of Bcl Xl mRNA in these two groups(P<0 01).The expression Fas/Apo 1 in leukemia was lower than that in normal (P<0.01). But between CR and NR, there was no difference with cytoimmune histiochemical stain and Westem blot .The antigen expression of P16 in leukemia was lower than that of normal person(P<0 01),also there was no statistical difference (P>0 05).Conclusions:The antigen expression of Bcl 2,Bcl 2 mRNA and Bcl Xl mRNA may be considered as prognostic indexes for AML.