Immunohistochemical analysis of TIMP-3 and MMP-9 in actinic keratosis,squamous cell carcinoma of the skin,and basal cell carcinoma |
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Authors: | Fabiano O. Poswar,Carlos A.C. Fraga,Lucyana C. Farias,John D. Feltenberger,Vitó ria P.D. Cruz,Sé rgio H.S. Santos,Christine M. Silveira,Alfredo M.B. de Paula,André L.S. Guimarã es |
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Affiliation: | 1. Department of Dentistry, Universidade Estadual de Montes Claros, Minas Gerais, Brazil;2. Department of Medicine, Universidade Estadual de Montes Claros, Minas Gerais, Brazil;3. School of Osteopathic Medicine, Touro University Nevada, NV, United States |
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Abstract: | The expression of metalloproteinases and their inhibitors has been related to different invasive and metastatic potentials in cancer. This study aims to investigate the immunohistochemical expression of TIMP-3 and MMP-9 in samples of basal cell carcinoma (BCC), squamous cell carcinoma of the skin (SCC), and actinic keratosis (AK). Immunohistochemistry was performed to evaluate the expression of TIMP-3 and MMP-9 in samples of BCC (n = 22), SCC (n = 10), and AK (n = 15). Ten fields of both tumor parenchyma and tumor stroma were photographed and counted in image software. The ratio of positive cells to total cells was used to quantify the staining. A higher expression of MMP-9 was found in tumor stroma of SCC compared to BCC and AK. No significant differences in TIMP-3 expression were observed among the groups. Considering the well-described differences between these neoplasms, these results provide additional evidence of the role of MMP-9 in tumor invasiveness of keratinocyte-derived tumors. |
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Keywords: | Basal cell carcinoma Squamous cell carcinoma Actinic keratosis Tissue inhibitor of metalloproteinase 3 Matrix metalloproteinase-9 |
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