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Carboplatin delays mammary cancer 4T1 growth in mice |
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| Authors: | Cristina Maria de Souza Conrado de Oliveira Gamba Cecília Bonolo de Campos Miriam Teresa Paz Lopes Mônica Alves Neves Diniz Ferreira Silvia Passos Andrade Geovanni Dantas Cassali |
| Institution: | 1. Department of General Pathology, Laboratory of Comparative Pathology, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;2. Department of Pharmacology, Laboratory of Antitumor Substances Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;3. Department of Physiology and Biophysics, Laboratory of Angiogenesis, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |
| Abstract: | Carboplatin is commonly used to treat a variety of tumors. We investigated the effects of carboplatin (100 mg/kg) in the development and metastatic dissemination of the 4T1 mice mammary carcinoma. Carboplatin was able to reduce tumor volume and the number of lung metastases in 50% compared to the control animals. Mitotic and apoptotic indices were also decreased by the treatment. Assessment of the vascularization of the tumors revealed a significant decrease in blood vessel formation by carboplatin. A decrease in nuclear positivity of CDC47 and cyclin D1 was observed in the group treated with carboplatin when compared to the control group. Positivity for p53 was observed in the control group (2/28; 5%) and the treated group (5/71; 4%). Carboplatin has been demonstrated to be an efficient regulator of 4T1MMT growth and dissemination. The action of this chemotherapeutic agent seems to be related to the induction of apoptosis and inhibition of angiogenesis and cell proliferation. |
| Keywords: | Mammary cancer model Carboplatin Cell proliferation Immunohistochemical |
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