重组蛋白ASP-1对高致病性禽流感M2e疫苗分子的佐剂活性

目的探讨重组蛋白ASP-1对高致病性禽流感M2e疫苗分子的免疫佐剂作用。方法在大肠杆菌原核表达系统中获得融合了pET32（＋）表达载体蛋白trxA和M2e三联体的重组蛋白TrxA-M2e3作为高致病性禽流感M2e疫苗分子;在大肠杆菌中截短表达盘尾丝虫活化相关蛋白ASP,获得截短片段ASP-1作为佐剂蛋白。将纯化后的重组蛋白TrxA-M2e3和ASP-1混合或者单独使用,免疫BALB/c小鼠后检测血清中M2e抗体水平。使用不同进化分支H5N1病毒对免疫小鼠进行致死性攻击,检测交叉免疫保护效果。结果获得了纯化的重组蛋白TrxA-M2e3和ASP-1;与TrxA-M2e3单独免疫组相比,应用佐剂蛋白ASP-1可显著提高免疫小鼠血清中M2e特异性IgG抗体滴度（P〈0.05）。在不同进化分支H5N1病毒的致死性攻击后,应用ASP-1与TrxA-M2e3联合免疫小鼠的肺组织病毒复制得到有效抑制,免疫小鼠全部存活。而其它免疫组小鼠全部死亡。结论 M2e是发展新型禽流感疫苗应对病毒变异的理想靶抗原分子,而重组蛋白ASP-1可以为一种新型佐剂在M2e疫苗分子诱导交叉免疫保护中发挥关键作用。

Adjuvanticity of recombinant protein ASP-1 on M2e-based vaccine against highly pathogenic avian influenza vaccine

In order to study the adjuvanticity of recombinant protein ASP-1 on M2e-based vaccine against highly pathogenic avian influenza,the recombinant protein TrxA-M2e3 serving as M2e-based vaccine against highly pathogenic avian influenza was expressed in prokaryotic system,and the vector protein trxA of pET32a(+) was fused to M2e3.The truncated segment ASP-1 of Onchocerca volvulus activation associated protein was expressed in E.coli as adjuvant protein.And the mice were vaccinated with purified TrxA-M2e3 and AS...