| 布鲁氏菌PCR鉴定方法的研究与应用 |
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| 引用本文： | 钟旗,易新萍,李博,吴冬玲,刘丽娅,叶锋,闫晶华,李金平,王力俭. 布鲁氏菌PCR鉴定方法的研究与应用[J]. 中国人兽共患病杂志, 2011, 27(3): 241-245 |
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| 作者姓名： | 钟旗 易新萍 李博 吴冬玲 刘丽娅 叶锋 闫晶华 李金平 王力俭 |
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| 作者单位： | 东北农业大学动物医学学院;新疆畜牧科学院兽医研究所;新疆天康畜牧生物技术股份有限公司;新疆动物卫生监督所; |
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| 基金项目： | 农业公益性行业科研专项经费(2008030261); 国家高技术研究发展计划(2007AA02Z405); 新疆维吾尔自治区高技术研究发展计划(200911109) |
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| 摘 要： | 目的采用PCR、PCR SSCP方法对布鲁氏菌进行快速鉴定，并对其种、型鉴别。方法分析已发表布鲁氏菌属、种、型基因序列，寻找出不同布鲁氏菌的种、型特异性碱基分布规律，设计特异性引物，用于布鲁氏菌属、种、型的鉴定；根据聚合酶链反应 单链构象多态性（PCR SSCP）分析原理，建立PCR SSCP方法，用于区分牛种A19疫苗株与其他型布鲁氏菌。结果所建立的PCR方法能高效、准确鉴定出布鲁氏菌，并能对其种、型进行区分；PCR SSCP方法可将A19疫苗株从其他型布鲁氏菌中区分出来。结论利用PCR、PCR SSCP方法能快速、准确地进行布鲁氏菌属、种、型以及疫苗株A19的鉴别，且简便、可靠，便于临床应用。
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| 关 键 词： | 布鲁氏菌 鉴定 PCR PCR-SSCP |
Study and application of polymerase chain reaction assay on Brucella identification |
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| ZHONG Qi,YI Xin-ping,LI Bo,WU Dong-ling,LIU Li-ya,YE Feng,YAN Jing-hua,LI Jin-ping,WANG Li-jian. Study and application of polymerase chain reaction assay on Brucella identification[J]. Chinese Journal of Zoonoses, 2011, 27(3): 241-245 |
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| Authors: | ZHONG Qi YI Xin-ping LI Bo WU Dong-ling LIU Li-ya YE Feng YAN Jing-hua LI Jin-ping WANG Li-jian |
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| Affiliation: | ZHONG Qi,YI Xin-ping,LI Bo,WU Dong-ling,LIU Li-ya,YE Feng,YNA Jing-hua,LI Jin-ping,WANG Li-jian(Institute of Veterinary Research,Xinjiang Academy of Animal Science,Urumqi 830000,China) |
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| Abstract: | The purpose of this study was to apply PCR and PCR-SSCP assay method to identify Brucella and specific type of Brucella.In order to analyze a great number of partition Brucella of types sequence which have been issued as specific genotype and search for the distributing role of genotype-specific nucleotides,the specific primers were designed and PCR method was applied to identify specific type of Brucella.PCR-SSCP method was established to distinguish vaccine strain A19 from other specific types of Brucella... |
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| Keywords: | Brucella identification PCR PCR-SSCP |
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