| 携带KGF基因重组腺病毒载体的构建与鉴定 |
| |
| 引用本文： | 贾庆华,哈小琴,吕同德,刘春杰. 携带KGF基因重组腺病毒载体的构建与鉴定[J]. 重庆医科大学学报, 2011, 36(1): 64-67 |
| |
| 作者姓名： | 贾庆华 哈小琴 吕同德 刘春杰 |
| |
| 作者单位： | 中国人民解放军兰州军区兰州总医院医学实验中心,甘肃省干细胞与基因药物重点实验室,兰州,730050 |
| |
| 摘 要： | 目的:构建携带角化细胞生长因子(Kemtinocyte growth factor,KGF)的腺病毒载体并观察其在细胞中的表达.方法:通过PCR从pcDNA3-KGFpIRES2-EGFP-KGF质粒中扩增出目的片段KGF,定向克隆至穿梭质粒载体pShuttle-CMV中,构建pShuttl-KGF.经酶切及测序鉴定后...
|
| 关 键 词： | 角化细胞生长因子 腺病毒 构建 表达 |
Construction and identification of recombinant adenovirus vector of keratinocyte growth factor |
| |
| JIA Qing-hua,et al. Construction and identification of recombinant adenovirus vector of keratinocyte growth factor[J]. Journal of Chongqing Medical University, 2011, 36(1): 64-67 |
| |
| Authors: | JIA Qing-hua et al |
| |
| Affiliation: | JIA Qing-hua,et al(Experimental Center of Medicine,Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA,Key Labaratory of Stem Cell and Gene Drug in Gansu Province) |
| |
| Abstract: | Objective:To construct and identify a recombinant adenovirus vector expressing keratinocyte growth factor(KGF) gene.Methods:KGF gene was amplified from the plasmid pIRES2-EGFP-KGF by polymerase chain reaction(PCR) and then inserted into the plasmid pShuttle-CMV to construct pShuttle-KGF.After being confirmed by restriction enzyme digestion and DNA sequencing,the DNA encoding KGF in the new structure was inserted into the vector of recombinant plasmid adenovirus and confirmed by restriction enzyme digestion.... |
| |
| Keywords: | keratinocyte growth factor(KGF) adenovirus construct expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
