解脲支原体血清8型感染小鼠盆腔炎症性疾病模型的建立

目的：建立解脲支原体阴道感染小鼠盆腔炎症性疾病的动物模型。方法：采用苯甲酸雌二醇预处理,给予Balb/c小鼠阴道接种解脲支原体血清8型感染液制成盆腔炎症性疾病模型,接种后1周及3周检测支原体后处死,取小鼠阴道、宫颈、子宫、输卵管组织常规病理包埋切片观察及检测支原体,并采用流式细胞术对外周血T淋巴细胞亚群进行了分析。结果：造模后小鼠阴道发红,有的分泌物增多,宫颈肥大,子宫及输卵管水肿。阴拭子培养支原体均为阳性。病理切片显示生殖器官4个部位均有明显中性粒细胞浸润。流式细胞术检测显示外周血CD4＋/CD8＋比值下降,具有统计学意义。结论：雌激素预处理下,解脲支原体阴道接种小鼠可逆行性感染引起宫颈炎、子宫炎、输卵管炎,机体免疫功能下降。此方法可成为盆腔炎症性疾病的成功造模方法之一。

Mice model of pelvic inflammatory disease inoculated by ureaplasma urealyticum

Objective:To study the mice model of pelvic inflammatory disease infected by ureaplasma urealyticum serotype 8.Methods:Oestradiaol-treated female BALB/c mice were inoculated intravaginally by serotype 8 of ureaplasma urealyticum.Ureaplasmas in the genital tracts were cultured 1 and 3 weeks after inoculation.Histopathologic changes of vagina,cervix,uterine and oviduct of mice were examined by light microscopy.Ureaplasma urealyticum were detected by nested polymerase chain reaction and T lymph cell of blood w...