| 5-FU、ADM联合X线外照射对乳腺癌MCF-7细胞Livin表达及凋亡的影响 |
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| 引用本文： | 周涛琪,张幸平,程丽,蒋骞,饶明月.5-FU、ADM联合X线外照射对乳腺癌MCF-7细胞Livin表达及凋亡的影响[J].重庆医科大学学报,2011,36(1):54-57. |
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| 作者姓名： | 周涛琪 张幸平 程丽 蒋骞 饶明月 |
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| 作者单位： | 重庆医科大学附属第一医院肿瘤科,重庆,400016 |
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| 摘 要： | 目的:观察抗肿瘤药物氟尿嘧啶(5-fluorouracil,5-FU)、阿霉素(Adriamycin,ADM)联合X线外照射对人乳腺癌MCF-7细胞Livin基因表达的干扰效应及对细胞凋亡的影响.方法:用MTT法分别测定5-FU、ADM 2种药物作用MCF-7细胞72 h的半数抑制浓度(Half maximal inh...
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| 关 键 词： | Livin基因 化疗药物 RT-PCR 免疫组化 放射增敏 |
Effect of 5-FU,ADM combined with X-ray irradiation on livin expression and apoptosis of breast cancer cell line MCF-7 |
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| ZHOU Tao-qi,et al.Effect of 5-FU,ADM combined with X-ray irradiation on livin expression and apoptosis of breast cancer cell line MCF-7[J].Journal of Chongqing Medical University,2011,36(1):54-57. |
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| Authors: | ZHOU Tao-qi |
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| Institution: | ZHOU Tao-qi,et al(Department of Oncology,the First Affiliated Hospital,Chongqing Medical University) |
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| Abstract: | Objective:To observe 5-FU,ADM combined with X-ray irradiation on interferring gene expression of Livin and apoptosis of human breast cancer cell line MCF-7.Methods:The IC50 of 5-FU,ADM were detected by MTT in the time of 72 h.The experiment had four groups:blank control group,irradiation group,5-FU + irradiation group,ADM + irradiation group.The Immunohistochemistry and RT-PCR were used to evaluate the Livin protein and mRNA expression,the apoptosis rate was analysed by flow cytometry.Results:Compare the bl... |
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| Keywords: | livin chemotherapeutics RT-PCR immunohistochemistry radiation sensitive |
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