细胞DNA定量分析技术在宫颈癌筛查中的应用

目的:比较和评估两种宫颈癌筛查方法的应用价值。方法:选取2010年1月至 2013年2月到漳浦县医院进行宫颈癌检查的病例14119例。均为宫颈刷取材，液基方法同时制两张片，一张进行巴氏染色，用于常规细胞学TBS诊断。另一张经Feulgen染色，用于细胞DNA定量分析检测。临床活检取材均在阴道镜指导下进行。结果:共建议活检671例。用于回顾性研究的388例有活检结果。其中有宫颈癌5例，CINⅢ/原位癌38例，44例CINⅡ及137例CINⅠ，其余164例为慢性宫颈炎。以活检结果≥CINⅡ作为宫颈癌前病变的阳性标准，采用细胞DNA定量分析检测的敏感性分别为70.11%与97.7%，而常规细胞学检测的敏感性分别为45.98%与58.62%。同一个标本同时采用两种方法联合检测，敏感性可提高到99.89%。结论:细胞DNA定量分析技术可作为宫颈癌及癌前病变检测的辅助手段。

The application of DNA-image cytometry in cervical cancer screening

Objective:To evaluate the application value of DNA image cytometry（DNA-ICM） in the screening of cervical carcinoma.Methods:All 14119 women screened from 2010 to 2013 were reviewed.The cytology samples were taken by cervix brush and prepared to 2 slides for each case:one was Pap-stained and read by cytologist according to the TBS criteria,the other was Feulgen-stained,scanned and analyzed by DNA-ICM.The biopsies were conducted with the guidance of colposcopy.Results:In the retrospective study,388 in 671 women recommended were reported with biopsy results which included 5 cases with cervical cancer,38 CINⅢ/CIS cases,44 CINⅡand 137 CINⅠ.The sensitivity of DNA-ICM were 70.11% and 97.7%,while the TBS report of cervical cytology test were 45.98% and 58.62%.The sensitivity increased to 99.89% when combining the both.Conclusion:DNA-ICM is a useful adjunct for cervical cancer screening combined with cervical cytology test.