交泰丸指纹图谱研究

目的:建立交泰丸的HPLC指纹图谱检测方法。方法:采用Agilent SB-AQ色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈-0.05 mol·L^-1磷酸二氢钾溶液(44:56)(每100 ml中加十二烷基硫酸钠0.4 g,以磷酸调节pH值为3.5),流速1.0 ml·min^-1,检测波长276 nm,柱温30 ℃,进样量5 μl。结果:通过"中药色谱指纹图谱相似度评价系统2004A版"分析确定了12个共有峰, 10批交泰丸的指纹图谱相似度均大于0.95。结论:该方法稳定可靠,为交泰丸质量标准建立提供了可靠的科学依据。

Study on HPLC fingerprints of Jiaotai bolus

OBJECTIVE To establish HPLC fingerprints of Jiaotai bolus. METHODS HPLC fingerprints were determined on Agilent ZORBAX SB-Aq column (4.6 mm × 250 mm, 5 μm); mobile phase: 0.05 mol· L^-1 of potassium dihydrogen phosphate and 0.4 g of sodium lauryl sulfate dissolved in 100 ml of a mixture of acetonitrile and water (44:56); pH value adjusted to 3.5 by phosphoric acid; flow rate at 1.0 ml· min^-1; column temperature at 30 ℃ and detection wavelength at 276 nm, and injection volume of 5 μl. Similarity evaluation system for chromatographic fingerprints of traditional Chinese medicine (2004A) was used for analysis. RESULTS Twelve common peaks were selected as fingerprint peaks of Jiaotai bolus, and 6 common peaks were determined by relative time of standard substance. The similarity of 10 batches of Jiaotai bolus were all more than 0.95.CONCLUSION The established HPLC fingerprint of Jiaotai bolus has desirable precision, reproducibility, and can provide a basis for quality evaluation of Jiaotai bolus.