液相色谱-质谱联用分析银线莲中六种核苷类物质

目的: 分析银线莲水提物中的胞苷、尿苷、腺嘌呤、肌苷、鸟苷、腺苷6种核苷类物质。方法: 高效液相色谱法－电喷雾电离/离子阱质谱法(HPLC-ESI/MS)对银线莲中超声波辅助萃取法水提物中6种核苷类物质进行测定和鉴别。结果: 银线莲药材中检测到胞苷、尿苷、腺嘌呤、肌苷、鸟苷和腺苷6种核苷成分。6种核苷类物质质量浓度在1.25~80.0 μg·ml^-1范围内呈良好线性关系(r≥0.999 4),回收率在92.7%~96.8%之间,RSD≤1.6%。测得银线莲药材提取物中胞苷、尿苷、腺嘌呤、肌苷、鸟苷和腺苷含量分别为0.106 2,0.339 1,0.069 9,0.082 0,0.030 8,0.107 4 mg·g^-1。结论: 此法简便、灵敏快速,首次报道银线莲中含有重要药理活性的6种核苷类物质,为银线莲的药理和临床试验研究提供理论基础。

Analysis of six nucleosides from Goodyera matsumurana by high performance liquid chromatogrpahy-mass spectrometry

OBJECTIVE To analyze the six nucleosides (cytidine, uridine, adenine, inosine, guanosine and adenosine) from aqueous extract of Goodyera matsumurana. METHODS The six nucleosides were extracted from Goodyera matsumurana by ultrasonic-assisted aqueous extraction, and were identified and determined by using high performance liquid chromatography-electrospray ionization/ion traps mass spectrometry (HPLC-ESI/MS). RESULTS Cytidine, uridine, adenine, inosine, guanosine and adenosine in Goodyera matsumurana were identified. The linearity was good within the concentration range of 1.25－80 μg·ml^-1 (r≥0.999 4) for the six nucleosides.The average recovery of them were 92.7%－96.8% with RSD≤1.6%. The content of six nucleosides (cytidine, uridine, adenine, inosine, guanosine and adenosine) from Goodyera matsumurana were 0.106 2 mg·g^-1, 0.339 1 mg·g^-1, 0.069 9 mg·g^-1, 0.082 0 mg·g^-1,0.030 8 mg·g^-1 and 0.107 4 mg·g^-1, respectively. CONCLUSION The proposed method is simple, accurate and rapid. So far there is no report indicating that Goodyera matsumurana contains these six important bioactive nucleoside compounds. It provides a scientific basis for further pharmacological and clinical studies on Goodyera matsumurana.