Molecular method for discrimination between Francisella tularensis and Francisella-like endosymbionts |
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| Authors: | Escudero Raquel Toledo A Gil Horacio Kovácsová Katarina Rodríguez-Vargas Manuela Jado Isabel García-Amil Cristina Lobo Bruno Bhide Mangesh Anda Pedro |
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| Institution: | Raquel Escudero, A. Toledo, Horacio Gil, Katarina Kovácsová, Manuela Rodríguez-Vargas, Isabel Jado, Cristina García-Amil, Bruno Lobo, Mangesh Bhide, and Pedro Anda |
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| Abstract: | Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Francisella-like endosymbionts that can produce a misleading positive result. A new, efficient molecular method for detection of Francisella tularensis and its discrimination from Francisella-like endosymbionts, as well as two variants associated with human disease (unusual F. tularensis strain FnSp1 and F. tularensis subsp. novicida-like strain 3523), is described. The method is highly specific and sensitive, detecting up to one plasmid copy or 10 genome equivalents. |
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