钙蛋白酶10基因多态性与贵阳地区人群2型糖尿病的相关性研究

目的探讨钙蛋白酶10(Calpain10)基因第3内含子SNP19多态性与贵阳地区人群2型糖尿病(T2DM)遗传易感性的关系。方法采用病例-对照研究设计,对74例T2DM患者和72例健康对照应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测Calpain10基因SNP19位点多态性,并与不同人群进行比较分析。结果与对照组相比病例组SNP19基因型频率、等位基因频率均无显著差异,与其他研究人群的结果相近。结论 Calpain10基因SNP19位点多态性可能与贵阳地区人群T2DM的发生无关。

Study on correlation between calpain 10 genetic polymorphism and type 2 diabetes mellitus in Guiyang area population

Objective To investigate the relation of SNP19 polymorphism of intron 3 of calpain 10 gene with the genetic susceptibility to type 2 diabetes mellitus in Goiyang area population. Methods The case-control design was adopted. PCR-RFI.P method was used to determine the distribution of allele and genotype frequencies of SNP 19 polymorphism （1/2） in intron 3 of calpain 10 gene in 74 patients with type 2 diabetes and 72 normal control subjects. The comparative analysis of the results in different populations was conducted. Results In two experiment design, the genotype and gene frequency between cases and controls had no significant difference（P〉0.05）. The result was similar to that of other research groups. Conclusion The occurrence of type 2 diabetes mellitus may be little associated with the SNP19 polymorphism of ealpain 10 gene in Guiyang area population.