| 经骨向诱导的骨髓间充质干细胞在脱钙骨上生长行为的实验研究 |
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| 引用本文： | 娄鸣,李晓红,饶国洲,朱勇,唐成芳,王峰,王湘.经骨向诱导的骨髓间充质干细胞在脱钙骨上生长行为的实验研究[J].中国美容医学,2009,18(9):1299-1302. |
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| 作者姓名： | 娄鸣 李晓红 饶国洲 朱勇 唐成芳 王峰 王湘 |
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| 作者单位： | 1. 西安医学院口腔系,陕西,西安,710021
2. 西安交通大学口腔医院,陕西,西安,710004 |
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| 基金项目： | 西安医学院科研基金( |
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| 摘 要： | 目的:建立成骨细胞-脱钙骨支架复合物,观察其诱导成骨能力,从而探寻组织工程化骨的体外构建方法.方法:运用细胞沉淀法将大鼠BMSCs诱导形成的成骨细胞接种于脱钙骨支架,通过扫描电镜(SEM)、免疫荧光观察脱钙骨表面细胞生长情况,通过上清液碱性磷酸酶(ALP)活性及Ⅰ型前胶原羧端肽(PICP)、骨钙素检测观察成骨细胞活性.结果:扫描电镜示成骨细胞在脱钙骨表面生长良好,上清液ALP、PICP及骨钙素表达均明显高于对照组(P＜0.05),并随时间的增长而显著增加(P＜0.05).结论:采用细胞沉淀法成功将成骨细胞接种于脱钙骨支架,成骨细胞在支架材料中增殖旺盛,细胞活性状态良好,具有良好的骨形成能力,脱钙骨支架可作为骨组织工程中载体支架的较优选择.
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| 关 键 词： | 骨髓间充质干细胞 脱钙骨支架 组织工程 |
Experimental study of bone-induced bone mesenchymal stem cells on decalcification bone scaffolds |
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| LOU Ming,LI Xiao-hong,RAO Guo-zhou,ZHU Yong,TANG Cheng-fang,WANG Feng,WANG Xiang.Experimental study of bone-induced bone mesenchymal stem cells on decalcification bone scaffolds[J].Chinese Journal of Aesthetic Medicine,2009,18(9):1299-1302. |
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| Authors: | LOU Ming LI Xiao-hong RAO Guo-zhou ZHU Yong TANG Cheng-fang WANG Feng WANG Xiang |
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| Institution: | 1.Department of Oral of Xi'an Medical College;Xi'an 710021;Shaanxi;China;2.The Stomatology Hospital of Xi'an Jiaotong University;Xi'an 710004;China |
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| Abstract: | Objiective To probe into the methods of tissue-engineering bone construction in vitro by building osteoblasts-decalcification bone scaffolds complex and observing its ability of inducing osteogenesis.Methods Inoculating the osteoblasts on the decalcification bone scaffold by precipitation method,and observing the cells growth by scanning electron microscope(SEM) and immunofluorescence,the osteogenic ability by detecting ALP,PICP(Procollagen type I C-terminal peptide) and Osteocalcin.Results SEM results show... |
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| Keywords: | bone mesenchymal stem cells decalcification bone scaffold tissue engineering |
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