| TGF-β_1、BMP-7 mRNA在实验性肝纤维化中的表达及意义 |
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| 引用本文： | 黄宁,曾令兰,李淑莉,苏凤菊. TGF-β_1、BMP-7 mRNA在实验性肝纤维化中的表达及意义[J]. 微循环学杂志, 2007, 17(3): 19-21,F0002 |
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| 作者姓名： | 黄宁 曾令兰 李淑莉 苏凤菊 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院传染病实验室,武汉,430022 |
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| 摘 要： | 目的:检测转化生长因子β1(TGF-β1)、骨形态发生蛋白-7(BMP-7)mRNA在实验性大鼠肝纤维化中的表达,并研究其与病程的关系。方法:采用复合因素建立肝纤维化动物模型,HE染色观察肝脏组织病理表现,实时荧光定量逆转录-聚合酶链反应技术(RealTimeRT-PCR)检测TGF-β1、BMP-7mRNA的表达水平,并分析两指标与病程的相关性。结果:肝纤维化动物TGF-β1mRNA明显高于正常对照组,而BMP-7mRNA水平明显低于正常对照组。随着病程的延长,TGF-β1mRNA表达水平呈明显上升趋势,而BMP-7mRNA则呈下降趋势。结论:肝纤维化的进程与TGF-β1mRNA水平升高和BMP-7mRNA表达下降有一定关系。
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| 关 键 词： | 肝纤维化 转化生长因子β1 骨形态发生蛋白-7 实时荧光定量逆转录-聚合酶链反应技术 大鼠 |
| 文章编号： | 1005-1740（2007）03-0019-03 |
| 收稿时间： | 2007-01-22 |
| 修稿时间： | 2007-01-222007-03-17 |
Expression and Significance of TGF-β1 and BMP-7 mRNA in Experimental Rat with Hepatic Fibrosis |
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| Huang Ning,Zeng Linglan,Li Shuli,et al. Expression and Significance of TGF-β1 and BMP-7 mRNA in Experimental Rat with Hepatic Fibrosis[J]. Chinese Journal of Microcirculation, 2007, 17(3): 19-21,F0002 |
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| Authors: | Huang Ning Zeng Linglan Li Shuli et al |
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| Affiliation: | Huang Ning,Zeng Linglan,Li Shuli,et al Department of Infection Disease,Union Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 320022 |
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| Abstract: | Objective:To investigate the expression and significance of transforming growth factor-beta1(TGF-β1),bone morphogentic protein-7(BMP-7)mRNA in experimental rat hepatic fibrosis.Method:To establish animal models of hepatic fibrosis induced by the exposure of rats to component factors.Pathological section examined the pathological changes of liver.Real time RT-PCR assessed expression of TGF-β1 and BMP-7 mRNA.Results:The expression of TGF-β1 mRNA in hepatic fibrosis rats was higher than in normal control rats,and the expression of BMP-7 mRNA was lower than in normal control rats.With the aggravation of the disease,TGF-β1 mRNA was apt to an obvious increase,while BMP-7mRNA showed an adverse change.Conclusion:The intensification of hepatic fibrosis was related to the high level of TGF-β1 mRNA and the low performance of BMP-7 mRNA. |
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| Keywords: | Hepatic fibrosis TGF-β1 BMP-7 Real Time RT-PCR Rat |
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