p27kip1基因对食管癌细胞DNA复制及蛋白质合成的影响

目的:研究p27kip1基因对人食管癌细胞EC-9706的DNA复制及蛋白质合成的影响。方法: 重组体腺病毒Ad-p27kip1转染EC-9706细胞, 采用细胞生长计数、 ³H]-TdR、³H]-Leucine掺入法、流式细胞术,研究其对食管癌细胞DNA复制及蛋白合成的影响。结果: Ad-p27kip1组细胞生长明显受抑, ³H]-TdR、³H]-Leucine掺入量均明显减少,与对照组比较P<0.01;食管癌细胞的增殖明显受抑制。结论: p27可有效抑制食管癌细胞的增殖活性,这与其抑制食管癌细胞DNA复制及蛋白质合成有关。

Effects of p27kip1 gene on DNA and protein synthesis in esophageal carcinoma cells

AIM: To investigate the effects of p27kip1 ge ne on DNA replication and protein synthesis in esophageal carcinoma cells. METHODS: Recombinant adenovirus Ad-p27kip1 was constructed and transf ected into esophageal carcinoma cell EC-9706, and its effects on DNA replication , protein synthesis and the growth of esophageal carcinoma cells were explored b y means of cell growth count, -TdR, -Leucine incorporation meth od and flow cytometry. RESULTS: The growth of esophageal carcino ma cells was inhibited obviously. The radioactive intensity of -TdR and -Leucine in Ad-p27kip1 group decreased significantly than that in contr ol group after EC-9706 transfection (P<0 01), while the multiplication of e sophageal carcinoma cell was inhibited obviously. CONCLUSION: Ad -p27kip1 inhibited the multiplication of the esophageal carcinoma cells, which m ay be related to its suppressive function for DNA replication and protein synthesis .