基于HPLC多指标成分测定及指纹图谱多模式识别的不同产地不同品种当归质量差异分析

目的 建立当归Angelicae sinensis的HPLC指纹图谱并测定其主要有效成分的含量,结合多种化学计量学分析,确定不同产地不同品种(岷归1号、岷归2号)当归差异成分,为当归药材质量控制提供参考。方法 采用HPLC法建立不同品种当归的指纹图谱,并对指标成分进行含量测定。采用相似度评价、聚类分析(cluster analysis,CA)、因子分析、主成分分析(principal component analysis,PCA)、正交偏最小二乘法判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)、Fisher线性判别分析进行数据分析。结果 HPLC指纹图谱共标定了不同品种当归中的16个共有峰,指认出绿原酸、阿魏酸、洋川芎内酯I、洋川芎内酯H、洋川芎内酯A、阿魏酸松柏酯、藁本内酯、丁烯基苯酞8个色谱峰。岷县产区当归的2个品种当归间存在差异的成分是绿原酸(P<0.01)、洋川芎内酯I(P<0.01)、洋川芎内酯H(P<0.01)、藁本内酯(P<0.01)和丁烯基苯酞(P<0.05),渭源产区的是洋川芎内酯I(P<0.01)、洋川芎内酯H(P<0.01)、洋川芎内酯A(P<0.01)、藁本内酯(P<0.01);临洮产区的是绿原酸(P<0.01)、阿魏酸(P<0.01)、阿魏酸松柏酯(P<0.01)、丁烯基苯酞(P<0.05);通渭产区的是阿魏酸(P<0.01)、洋川芎内酯H(P<0.05)、洋川芎内酯A(P<0.05)、藁本内酯(P<0.01)。结论 建立的HPLC指纹图谱结合含量测定、CA、因子分析、PCA、OPLS-DA分析可以客观、全面、有效地确定不同产地不同品种当归中主要有效成分的差异,可为当归品种鉴别及质量控制提供有力支撑。

Quality differences of different origins and varieties of Angelicae sinensis based on HPLC multi-index composition determination and multi-pattern recognition method of fingerprint

Objective The HPLC fingerprint ofAngelicae sinensis was established and the content of its main effective components was determined.Combined with a variety of stoichiometric analyses,the different components of different varieties (Mingui I and Mingui II) ofA.sinensis were determined to provide a reference for its quality control. Methods HPLC method was used to establish the fingerprint of different varieties ofA.sinensis and determine the content of index components.The data were analyzed by similarity evaluation combined with cluster analysis (CA),factor analysis,principal component analysis (PCA),orthogonal partial least squares discriminant analysis (OPLS-DA),Fisher linear discriminant (FLDA).Results A total of 16 common peaks inA.sinensis from different origins were calibrated by HPLC fingerprint.Eight chromatographic peaks of chlorogenic acid,ferulic acid,senkyunolide I,senkyunolide H,senkyunolide A,coniferyl ferulate,ligustilide,and butenyl phthalide were identified.The differences between the two varieties ofA.sinensis in the Min County were chlorogenic acid (P<0.01),senkyunolide I (P<0.01),senkyunolide H (P<0.01),ligustilide (P<0.01)) and butenyl phthalide (P<0.01),in Weiyuan,were senkyunolide I (P<0.01),senkyunolide H (P<0.01),senkyunolide A (P<0.01),ligustolide (P<0.01);In Lintao werechlorogenic acid (P<0.01),ferulic acid (P<0.01),coniferyl ferulate (P<0.01),butenyl phthalide (P<0.05);In Tongwei were ferulic acid (P<0.01),senkyunolide H (P<0.05),senkyunolide A (P<0.05),and ligustolide (P<0.01).Conclusion The established HPLC fingerprint combined with content determination,CA,factor analysis,PCA,OPLS-DA can objectively,comprehensively,and effectively determine the differences of main effective components in different varieties ofA.sinensis,and it can provide strong support for the variety identification and quality control ofA.sinensis.