天麻钩藤饮联合针刺对自发性高血压大鼠的降压作用及机制研究

目的研究天麻钩藤饮联合针刺对于自发性高血压大鼠(SHR)的降压效果及作用机制。方法使用随机数字表法将40只SHR分为4组:针刺组、天麻钩藤饮组、针刺+天麻钩藤饮组、模型组,每组10只。检测治疗前后各组大鼠的血压、神经肽Y(NPY)和内皮素-1(ET-1)水平。结果①血压变化方面:治疗2周、治疗4周时,针刺+天麻钩藤饮组较针刺组、天麻钩藤饮组血压下降更明显,差异均有统计学意义(P<0.05);治疗2周时,天麻钩藤饮组、针刺+天麻钩藤饮组较模型组血压下降更明显,差异均有统计学意义(P<0.05);治疗4周时,针刺组、天麻钩藤饮组、针刺+天麻钩藤饮组较模型组血压下降更明显,差异均有统计学意义(P<0.05)。②NPY及ET-1变化方面:针刺+天麻钩藤饮组NPY、ET-1水平较针刺组、天麻钩藤饮组降低更明显,差异均有统计学意义(P<0.05);针刺组、天麻钩藤饮组、针刺+天麻钩藤饮组NPY水平较模型组降低更明显,差异均有统计学意义(P<0.05);天麻钩藤饮组、针刺+天麻钩藤饮组ET-1水平较模型组降低更明显,差异均有统计学意义(P<0.05)。结论天麻钩藤饮联合针刺对于高血压的降压作用比单独应用针刺或天麻钩藤饮更加显著,两者联合应用可能存在协同效应,这种效应的发挥可能与血清NPY和ET-1的降低有关。

Antihypertensive Effect and Mechanism of Tianma Gouteng Yin Combined with Acupuncture on Spontaneously Hypertensive Rats

Objective To study the antihypertensive effect and mechanism of Tianma Gouteng Yin combined with acupuncture on spontaneously hypertensive rats(SHRs).Methods Forty SHRs were divided into four groups by random number table method:acupuncture group,Tianma Gouteng Yin group,acupuncture+Tianma Gouteng Yin group,model group,with 10 rats in each group.Blood pressure,neuropeptide Y(NPY),and endothelin-1(ET-1)were measured.Results①Blood pressure changes:at 2 and 4 weeks of treatment,the decrease of blood pressure in acupuncture+Tianma Gouteng Yin group was more obvious than those in acupuncture group and Tianma Gouten yin group(P<0.05);At 2 weeks of treatment,blood pressure of Tianma Gouten Yin group and acupuncture+Tianma Gouten Yin group decreased more obviously than that of model group(P<0.05);At 4 weeks of treatment,blood pressure of acupuncture group,Tianma Gouten Yin group and acupuncture+Tianma Gouten Yin group decreased more obviously than that of model group(P<0.05).②NPY and ET-1:the levels of NPY and ET-1 in acupuncture+Tianma Gouten Yin group were significantly lower than those in acupuncture group and Tianma Gouten Yin group(P<0.05);The levels of NPY in acupuncture group,Tianma Gouten Yin group,and acupuncture+Tianma Gouten Yin group were significantly lower than that in model group(P<0.05);The levels of ET-1 in Tianma Gouten Yin group and acupuncture+Tianma Gouten Yin group were significantly lower than that in model group(P<0.05).Conclusion The antihypertensive effect of Tianma Gouteng Yin combined with acupuncture on hypertension is more significant than that of single application.The combined application of Tianma Gouteng Yin and acupuncture has synergistic effect,which may be related to the decrease of serum NPY and ET-1.