Sustainable succinic acid production from rice husks |
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Authors: | Daiane B Bevilaqua Sheila Montipó Giovanni B Pedroso Ayrton F Martins |
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Institution: | Chemistry Department, Federal University of Santa Maria, CEP 97105-900 Santa Maria, RS, Brazil |
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Abstract: | In this study, an investigation was carried out into the production of succinic acid by Actinobacillus succinogenes using the residual biomass rice husks as a sustainable carbon source. The rice husks were submitted to acid hydrolysis in autoclave and in a pressurized polytetrafluorethylene vessel. The hydrolysis conditions were optimized with the aid of a factorial design. The best results were obtained with a pressurized reactor using HCl 2.2% (v v?1), at a temperature of 174 °C (59 bar), 46 min reaction time, and producing 19.0 g L?1 glucose and 3.01 g L?1 xylose. The hydrolysate was detoxified through a combination of pH regulation and adsorption on active carbon; it was subsequently, fermented in anaerobic medium at 37 °C; the nutrient concentration and the agitation speed were also optimized by factorial design. After 54 h static fermentation of the rice husks hydrolysate, supplemented with 8.40 g L?1 yeast extract and 1.40 g L?1 NaHCO3, an amount of 12.5 g succinic acid L?1 was produced, which corresponds to a yield of 59.9%. This confirms that, rice husks can definitely be used as substrate to produce succinic acid and other priority chemicals |
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Keywords: | Sustainable succinic acid production Rice husks Pressurized acid hydrolysis Multivariate optimization |
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