细胞因子对培养的人视网膜色素上皮细胞胶原合成的影响

目的 观察转化生长因子β(transforming growth factor-beta,TGF-β)和干扰素γ(interferon-gamma,IFN-γ)对人视网膜色素上皮细胞(retinal pigment epithelial cells,RPE)胶原合成的影响。 方法 用³Ｈ-脯氨酸掺入、免疫细胞化学及分子杂交技术观察TGF-β和IFN-γ分别及联合作用下培养的人RPE胶原合成活性。 结果 TGF-β在10 ng/ml浓度使RPE对³Ｈ-脯氨酸的摄取增加130.87％，并增强Ⅳ、Ⅰ和Ⅲ型胶原荧光染色和mRNA表达。IFN-γ在10 000 U/ml浓度抑制54.72％的脯氨酸摄取，并减弱Ⅳ型胶原染色和3种胶原的mRNA表达。 结论 TGF-β和IFN-γ对RPE的胶原合成分别具有正向及负向调控作用，IFN-γ可能用于抑制RPE胶原合成。 (中华眼底病杂志, 1999, 15: 245-248)

Effects of cytokines on collagen synthesis in human retinal pigment epithelial cells

Objective To investigate the effects of transformin growth factor-beta (TGF-β) and interferon-gamma(IFN-γ)on collagen synthesis in human retinal pigment epithelial cells(RPE)． Methods TGF-β(0.01～10 ng/ml),recombinant IFN-γ(100～10000 Ｕ/ml)or a combination of two were added to cultures of RPE and collagen synthesis of the cells were measured by³ H-proline incorporation assay,indirect immunofluorescence staining and dot-blot hybridization. Results TGF-β at 10 ng/ml increased cell uptake of ³ H-proline to 130．87% of controls．It intensified Type IV,I and Ⅲ collagen fluorescent staining as well as mRNA expression．IFN-γ at 10000　Ｕ/ml caused 54．72% inhibition of ³ H-proline uptake by RPE,and decreased TypeⅣ collagen fluorescent staining as well as mRNA expression of Type Ⅳ,I and Ⅲ collagens. Conclusion TGF-β and IFN-γ stimulated and inhibited collagen synthesis of human RPE,respectively．The combination of two had antagonistic effects．IFN-γ can be used for inhibition of collagen synthesis of RPE. (Chin J Ocul Fundus Dis, 1999, 15: 245-248)