肿瘤坏死因子-α基因启动子多态性与慢性乙型肝炎易感性的关系

目的探讨湖北汉族人肿瘤坏死因子-α(TNF-α)基因启动子多态性及其与慢性乙型肝炎易感性之间的关系。方法应用聚合酶链反应-限制性片段长度多态性分析方法(RFLP),检测126名正常对照者和131例慢性乙型肝炎患者TNF-α基因启动子多态性。结果共发现12种启动子基因型,以GG.GG.CC.CC、GG.GG.CC.CA、GG.GG.CT.CC和GG.GA.CC.CC基因型多见,约占85%;通过对TNF-α基因启动子4个位点基因型分析发现,慢性乙型肝炎患者和正常对照者TNF-α基因启动子-238G/A、-857C/T位点基因型分布频率差异无显著性,而-308G/A、-863C/A位点基因型分布频率差异有显著性。结论湖北汉族人慢性乙型肝炎易感性与TNF-α基因启动子-308G/A、-863C/A位点多态性有关,其中TNF-α-308GA、-863CA基因型携带者患慢性乙型肝炎风险相对较小。

Polymorphism of Tumor Necrosis Factor-α Gene Promoter in Chinese Han People and Its Association with Susceptibility to Chronic Hepatitis B

Objective: To investigate the polymorphism of tumor necrosis factor-α(TNF-α) gene promoters in Chinese Han people, and to disclose whether such polymorphism is associated with susceptibility to chronic hepatitis B.Method: After process of extracting genomic DNA from blood of 126 control subjects and 131 patients with chronic hepatitis B, four single nucleotide polymorphism(SNP)sites in TNF-α gene promoter marked as-238G/A,-308G/A,-857C/T and-863C/A were determined by polymerase chain reaction(PCR)-restriction fragment length polymorphism(RFLP)analysis.Results: Twelve different promoter genotypes were detected from all of 257 research subjects, and GG·GG·CC·CC, GG·GG·CC·CA, GG·GG·CT·CC, GG·GA·CC·CC genotypes accounted for about 85% of genotypes in these subjects. By analyzing the four promoter genotypes of TNF-α, the results showed that -238G/A and-857C/T SNP sites polymorphisms were not associated with susceptibility to chronic hepatitis B, whereas the genotypes of-308G/A and-863C/A SNP sites were associated with susceptibility to chronic hepatitis B in Chinese Han people.Conclusion: There is an association between polymorphisms of the promoter region-308G/A and-863C/A of TNF-α and chronic hepatitis B. Subjects with TNF-α-308GA,-863CA genotypes may be resistant to HBV infection, which gives some new clues to the study of pathogenesis of chronic hepatitis B.