胶原三维立体培养模型中胚肺成纤维细胞与肺癌GLC-82细胞的相互作用

背景:组织细胞、炎细胞可能在肿瘤细胞的侵袭、转移中发挥着重要作用,而细胞间的相互作用对基质金属蛋白酶有影响.目的:观察胚肺成纤维细胞与肺癌GLC-82细胞在胶原三维立体培养条件下,相互作用对基质金属蛋白酶1,2,9表达的影响.方法:采用三维胶原立体培养模型,按比例混合胶原,4倍DMEM和灭菌水,1倍DMEM的液体胶原,其中胶原终浓度为0 75 g/mL.将GLC-82细胞、胚肺成纤维细胞分别单独培养及GLC-82细胞和胚肺成纤维细胞按5:1混合培养,待胶原固化后加入1倍DMEM培养基,48 h后收集上清液.Western blot法检测基质金属蛋白酶1表达水平,明胶酶谱法测定基质金属蛋白酶2,9的表达.结果与结论:胚肺成纤维细胞与肺癌GLC-82细胞混合培养组基质金属蛋白酶1及基质金属蛋白酶2,9分泌量大于单独培养组(P<0 05).结果提示,三维立体培养条件下,胚肺成纤维细胞与肺癌GLC-82细胞相互作用能通过上调基质金属蛋白酶1,2,9的表达和活化,促进肺癌侵袭和转移.

Interaction between lung fibroblast and lung cancer GLC-82 cells in a three-dimensional collagen culture model

BACKGROUND:The histocyte and inflammatory cells played an important role in the invasion and metastasis of lung cancer cells.However,cell interaction influenced matrix metalloproteinase.OBJECTIVE:To observe the influence of interaction between fetal lung fibroblasts(HFL) and lung cancer cells on matrix metalloproteinase-1,2,and 9(MMP-1,2,9) expression in three-dimensional co-culture.METHODS:Human lung cancer cells(GLC-82) and HFL were co-cultivated in three-dimensional collagen gels,which were mixed with co...